biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
B13-DE1, monoclonal
form
lyophilized
packaging
pkg of 100 μg
manufacturer/tradename
Roche
isotype
IgG1
storage temp.
2-8°C
General description
抗荧光素抗体。
该抗荧光素抗体结合荧光素标记样品后，可采用抗小鼠Ig荧光素/酶偶联物通过一步法检测，也可先用抗小鼠Ig荧光素，再用抗荧光素/酶偶联物通过二步法检测。
该抗体不含任何其他蛋白，因此可用于包被和标记。
内容
冻干品，稳定
该抗荧光素抗体结合荧光素标记样品后，可采用抗小鼠Ig荧光素/酶偶联物通过一步法检测，也可先用抗小鼠Ig荧光素，再用抗荧光素/酶偶联物通过二步法检测。
该抗体不含任何其他蛋白，因此可用于包被和标记。
内容
冻干品，稳定
Application
在下列方法中，使用抗荧光素抗体检测荧光素标记化合物：
- ELISA
- 免疫组织化学
- 原位杂交
- 蛋白质印迹法
Biochem/physiol Actions
该单克隆抗体与游离和结合的荧光素反应。
Preparation Note
工作浓度：结合物的工作浓度取决于应用类型和底物。以下浓度可作为指导：
工作溶液：磷酸盐缓冲液，pH 7.4
如改用水配制溶液，抗体会形成沉淀。
- ELISA：2-4 μg/ml
- 免疫组织细胞化学：0.5-2 μg/ml
- 原位杂交：0.2-0.4 μg/ml
- 蛋白质印迹：0.5-2 μg/ml
工作溶液：磷酸盐缓冲液，pH 7.4
如改用水配制溶液，抗体会形成沉淀。
加入1 mL双蒸水至最终浓度100 μg/ml。
使用前再复溶（重悬）15分钟。
使用前再复溶（重悬）15分钟。
Other Notes
仅用于生命科学研究。不用于诊断操作。
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Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
低风险生物材料
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