推荐产品
生物来源
mouse
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
B13-DE1, monoclonal
形式
lyophilized
包装
pkg of 100 μg
制造商/商品名称
Roche
同位素/亚型
IgG1
储存温度
2-8°C
一般描述
抗荧光素抗体。
该抗荧光素抗体结合荧光素标记样品后,可采用抗小鼠Ig荧光素/酶偶联物通过一步法检测,也可先用抗小鼠Ig荧光素,再用抗荧光素/酶偶联物通过二步法检测。
该抗体不含任何其他蛋白,因此可用于包被和标记。
内容
冻干品,稳定
该抗荧光素抗体结合荧光素标记样品后,可采用抗小鼠Ig荧光素/酶偶联物通过一步法检测,也可先用抗小鼠Ig荧光素,再用抗荧光素/酶偶联物通过二步法检测。
该抗体不含任何其他蛋白,因此可用于包被和标记。
内容
冻干品,稳定
特异性
该单克隆抗体与游离和结合的荧光素反应。
应用
在下列方法中,使用抗荧光素抗体检测荧光素标记化合物:
- ELISA
- 免疫组织化学
- 原位杂交
- 蛋白质印迹法
制备说明
工作浓度:结合物的工作浓度取决于应用类型和底物。以下浓度可作为指导:
工作溶液:磷酸盐缓冲液,pH 7.4
如改用水配制溶液,抗体会形成沉淀。
- ELISA:2-4 μg/ml
- 免疫组织细胞化学:0.5-2 μg/ml
- 原位杂交:0.2-0.4 μg/ml
- 蛋白质印迹:0.5-2 μg/ml
工作溶液:磷酸盐缓冲液,pH 7.4
如改用水配制溶液,抗体会形成沉淀。
重悬
加入1 mL双蒸水至最终浓度100 μg/ml。
使用前再复溶(重悬)15分钟。
使用前再复溶(重悬)15分钟。
其他说明
仅用于生命科学研究。不用于诊断操作。
警示用语:
Warning
危险声明
危险分类
Aquatic Chronic 3 - Skin Sens. 1
WGK
WGK 2
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
常规特殊物品
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