biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
B13-DE1, monoclonal
form
lyophilized
packaging
pkg of 100 μg
manufacturer/tradename
Roche
isotype
IgG1
storage temp.
2-8°C
General description
抗荧光素抗体。
该抗荧光素抗体结合荧光素标记样品后，可采用抗小鼠Ig荧光素/酶偶联物通过一步法检测，也可先用抗小鼠Ig荧光素，再用抗荧光素/酶偶联物通过二步法检测。
该抗体不含任何其他蛋白，因此可用于包被和标记。
内容
冻干品，稳定
该抗荧光素抗体结合荧光素标记样品后，可采用抗小鼠Ig荧光素/酶偶联物通过一步法检测，也可先用抗小鼠Ig荧光素，再用抗荧光素/酶偶联物通过二步法检测。
该抗体不含任何其他蛋白，因此可用于包被和标记。
内容
冻干品，稳定
Application
在下列方法中，使用抗荧光素抗体检测荧光素标记化合物：
- ELISA
- 免疫组织化学
- 原位杂交
- 蛋白质印迹法
Biochem/physiol Actions
该单克隆抗体与游离和结合的荧光素反应。
Preparation Note
工作浓度：结合物的工作浓度取决于应用类型和底物。以下浓度可作为指导：
工作溶液：磷酸盐缓冲液，pH 7.4
如改用水配制溶液，抗体会形成沉淀。
- ELISA：2-4 μg/ml
- 免疫组织细胞化学：0.5-2 μg/ml
- 原位杂交：0.2-0.4 μg/ml
- 蛋白质印迹：0.5-2 μg/ml
工作溶液：磷酸盐缓冲液，pH 7.4
如改用水配制溶液，抗体会形成沉淀。
加入1 mL双蒸水至最终浓度100 μg/ml。
使用前再复溶（重悬）15分钟。
使用前再复溶（重悬）15分钟。
Other Notes
仅用于生命科学研究。不用于诊断操作。
未找到合适的产品？
试试我们的产品选型工具.
signalword
Warning
hcodes
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 2
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
低风险生物材料
常规特殊物品
此项目有
Ignacio E Schor et al.
Current biology : CB, 28(22), 3547-3561 (2018-11-06)
Long non-coding RNAs (lncRNAs) can often function in the regulation of gene expression during development; however, their generality as essential regulators in developmental processes and organismal phenotypes remains unclear. Here, we performed a tailored investigation of lncRNA expression and function
J Spielmann et al.
BMC obesity, 4, 24-24 (2017-07-12)
Obesity was identified as a major risk factor for malignant diseases, but underlying mechanisms remain unclear. Natural killer (NK) cells, a pivotal aspect of innate immunity, are capable of identifying and killing virally infected and tumor cells. Previous studies have
Comparing nucleic acid lateral flow and electrochemical genosensing for the simultaneous detection of foodborne pathogens.
Aissa A B, et al.
Biosensors And Bioelectronics, 88, 265-272 (2017)
Yufeng Liu et al.
Oncology reports, 44(2), 499-508 (2020-07-07)
Apurinic/apyrimidinic endonuclease 1 (APE1) is a primary nuclear‑localized multifunctional protein in osteosarcoma. However, the cytoplasmic localization of APE1 was found to be functional and to increase with cisplatin resistance, yet the molecular mechanism is unknown. In the present study, we explored
Gerard Terradas et al.
G3 (Bethesda, Md.), 12(1) (2021-11-19)
Gene drives are programmable genetic elements that can spread beneficial traits into wild populations to aid in vector-borne pathogen control. Two different drives have been developed for population modification of mosquito vectors. The Reckh drive (vasa-Cas9) in Anopheles stephensi displays
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持