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Merck
CN

11426320001

Roche

抗-荧光素

from mouse IgG1 (clone B13-DE1)

别名:

抗体

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About This Item

UNSPSC代码:
12352200

生物来源

mouse

偶联物

unconjugated

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

B13-DE1, monoclonal

表单

lyophilized

包装

pkg of 100 μg

制造商/商品名称

Roche

同位素/亚型

IgG1

储存温度

2-8°C

一般描述

抗荧光素抗体。
该抗荧光素抗体结合荧光素标记样品后,可采用抗小鼠Ig荧光素/酶偶联物通过一步法检测,也可先用抗小鼠Ig荧光素,再用抗荧光素/酶偶联物通过二步法检测。
该抗体不含任何其他蛋白,因此可用于包被和标记。

内容
冻干品,稳定

特异性

该单克隆抗体与游离和结合的荧光素反应。

应用

在下列方法中,使用抗荧光素抗体检测荧光素标记化合物:
  • ELISA
  • 免疫组织化学
  • 原位杂交
  • 蛋白质印迹法
抗荧光素抗体已用于在四重标记PCR和核酸侧流分析中捕获扩增子,用于同时检测食源性致病菌。

制备说明

工作浓度:结合物的工作浓度取决于应用类型和底物。以下浓度可作为指导:
  • ELISA:2-4 μg/ml
  • 免疫组织细胞化学:0.5-2 μg/ml
  • 原位杂交:0.2-0.4 μg/ml
  • 蛋白质印迹:0.5-2 μg/ml

工作溶液:磷酸盐缓冲液,pH 7.4
如改用水配制溶液,抗体会形成沉淀。

重悬

加入1 mL双蒸水至最终浓度100 μg/ml。
使用前再复溶(重悬)15分钟。

其他说明

仅用于生命科学研究。不用于诊断操作。

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象形图

Exclamation mark

警示用语:

Warning

危险声明

危险分类

Aquatic Chronic 3 - Skin Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 2

闪点(°F)

does not flash

闪点(°C)

does not flash

法规信息

常规特殊物品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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