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Merck
CN

11418165001

Roche

生物素蛋白标记试剂盒

sufficient for 5 labeling reactions, kit of 1 (5 components), suitable for immunoblotting, suitable for ELISA

别名:

生物素, 蛋白质标记

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About This Item

UNSPSC代码:
12352200

用途

sufficient for 5 labeling reactions

质量水平

包装

kit of 1 (5 components)

制造商/商品名称

Roche

技术

ELISA: suitable
immunoblotting: suitable

储存温度

2-8°C

一般描述

用生物素标记蛋白。
待标记蛋白的游离氨基酸可通过形成稳定的氨基键,与D-生物素基--ε-氨基己酸-N-羟基-琥珀酰亚胺酯(生物素-7-NHS)反应。在Sephadex G-25柱上对未反应的生物素-7-NHS进行分离。

应用

生物素蛋白标记试剂盒用于生产变性的生物素化牛血清白蛋白(d-BSA),以包覆碲化镉(CdTe)量子点(QD)缀合物。
该试剂盒适用于通过生物素标记蛋白进行免疫化学、免疫印迹和ELISA。

包装

1个试剂盒包含5种组分。

质量

功能测试。

制备说明

工作溶液:溶液制备
  • 封闭液:将封闭剂瓶(绿色螺旋盖)中的内容物溶于300 ml双蒸水中。
  • PBS溶液:将PBS瓶(蓝色螺旋盖)中的内容物溶于300 l双蒸水中。
  • 生物素-7-NHS溶液:溶液应在标记前现配。加到包含250μI生物素-7-NHS DMSO溶液的反应样品瓶的内容物中,并将溶液涡旋数次。得到的生物素-7-NHS溶液浓度为20 mg/ml。对于标记混合物,生物素-7-NHS溶液的体积不应超过总反应体积的5%(例如,最多可向1 ml蛋白溶液中加50 μl生物素-7-NHS溶液)。

其他说明

仅用于生命科学研究。不可用于诊断。

仅试剂盒组分

产品编号
说明

  • Blocking Reagent (green screw cap)

  • Phosphate Buffered Saline (PBS) (blue screw cap)

  • D-Biotinoyl-ε-aminocaproic acid-N-hydroxysuccinimide ester (Biotin-7-NHS)

  • Dimethylsulfoxide (DMSO) (red screw cap)

  • Sephadex G-25 Columns

WGK

WGK 1

法规信息

含少量动物源组分生物产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

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