产品名称
生物素-16-UTP, pkg of 25 μL (250 nmol; 10mM)
assay
97.7% (HPLC)
form
solution
mol wt
Mr 987.5 (biotin-16-UTP-Li4)
packaging
pkg of 25 μL (250 nmol; 10mM)
manufacturer/tradename
Roche
color
colorless
solubility
water: miscible
shipped in
dry ice
storage temp.
−20°C
Quality Level
Analysis Note
典型分析：至少85％生物素-16-UTP（HPLC，面积％）。
Application
生物素标记的RNA可作为杂交探针用于：
- Northern 印迹
- Southern 印迹
- 菌落/菌斑转移筛选
- RNase保护实验
- 原位杂交
- 芯片杂交
General description
生物素-16-UTP中的生物素通过酰胺键与尿苷三磷酸结合。生物素-16-UTP是SP6、T3和T7 RNA聚合酶的底物。它可以取代体外转录反应中的UTP，用于RNA标记。利用ATP、GTP、CTP、UTP和生物素-16-UTP和相应的RNA聚合酶分别对具有T7、SP6或T3启动子的线性模板DNA进行体外转录。
Other Notes
仅用于生命科学研究。不用于诊断操作。
Physical form
生物素-16-UTP，四锂盐，10mM溶液
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
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