biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
clone 1.71.256, monoclonal
form
lyophilized
packaging
pkg of 100 μg
manufacturer/tradename
Roche
technique(s)
ELISA: 2-4 μg/mL, immunocytochemistry: 0.5-2 μg/mL, immunohistochemistry: 0.5-2 μg/mL, western blot: 0.5-2 μg/mL
isotype
IgG1κ
storage temp.
2-8°C
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General description
内容物：冻干粉
地高辛是一种半抗原，可用于核酸标记和检测系统。用地高辛标记的探针具有比放射性探针更高的灵敏度，从而可以实现更快的检测。它的危害性更低，并具有更长的保质期。
Application
使用抗地高辛抗体检测地高辛标记化合物，使用：
- 酶联免疫吸附测定（ELISA）
- 免疫组织细胞化学
- 原位杂交
- 免疫印迹
- 免疫荧光染色
- FISH（荧光 原位杂交 ）
Biochem/physiol Actions
单克隆抗体与游离和结合的地高辛反应。
在Na+、Mg2+和腺苷三磷酸(ATP)的存在下，地高辛可抑制钠离子泵。
Features and Benefits
- 游离和结合地高辛的特异性
- 抗体不受蛋白稳定，因此适合作为夹心 ELISA 的包被抗体，并用于标记程序。例如可与酶或荧光染料偶联，直接检测地高辛标记的样品。
Preparation Note
工作浓度： 抗体的工作浓度取决于应用和底物。以下浓度应作为指导：
工作溶液： 用于涂层应用
磷酸盐缓冲盐水，pH 7.4
- ELISA：用于包被：2～4μg/ml
- 免疫组织细胞化学：0.5～2μg/ml
- 原位杂交 ：0.2～0.4μg/ml
- 免疫印迹：0.5～2μg/ml
工作溶液： 用于涂层应用
磷酸盐缓冲盐水，pH 7.4
冻干抗体在+2至+8°C下稳定。复溶的抗体溶液在+2至+8°C下可稳定存放6个月。溶液可分装存放于-15至-25°C下。避免反复冻融。
加入 1 mL 双蒸水至终浓度 100μg/ml。
Analysis Note
无已知的交叉反应。
Other Notes
仅用于生命科学研究。不可用于诊断。
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Warning
hcodes
Hazard Classifications
Skin Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
does not flash
flash_point_c
does not flash
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商品
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
地高辛（DIG）标记方法和试剂盒适用于DNA和RNA DIG探针、随机引物DNA标记、切口平移标记、5'和3'寡核苷酸末端标记。
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