产品名称
抗地高辛-罗丹明,Fab片段, from sheep
biological source
sheep
conjugate
rhodamine conjugate
antibody form
purified antibody
antibody product type
primary antibodies
clone
polyclonal
form
lyophilized (clear, red solution after reconstitution)
packaging
pkg of 200 μg
manufacturer/tradename
Roche
isotype
IgG
storage temp.
2-8°C
Quality Level
Application
使用抗地高辛-罗丹明Fab片段检测地高辛标记的化合物：
- 在糖缀合物研究中使用地高辛标记糖
- 在原位杂交中使用荧光
- 免疫组织细胞化学
- 原位杂交
- 脱氧核糖核酸纤维分析
Biochem/physiol Actions
绵羊的多克隆抗体显示出与地高辛和地高辛100%的反应性，但与其他类固醇如人雌激素（如雌二醇）或雄激素（如睾酮）没有交叉反应性。
General description
地高辛是一种半抗原，可用于核酸标记和检测系统。本产品包含多克隆抗地高辛抗体的Fab片段，与罗丹明偶联。
Other Notes
仅用于生命科学研究。不可用于诊断。
Preparation Note
加入1ml双蒸水至终浓度为200μg/ml。
地高辛免疫后，用离子交换色谱法纯化绵羊IgG，用免疫吸附法分离特异性IgG。木瓜蛋白酶消化获得的Fab片段通过凝胶过滤纯化，与特定标记缀合，并稳定在缓冲液中。
工作浓度：共轭物的工作浓度取决于应用和底物。以下浓度应作为指导：糖缀合物中地高辛标记糖的检测：20至50 & # 956；g/ml
工作溶液：PBS，0.5%牛血清白蛋白，pH 7.4。
1%封闭剂（w/v）、1-5%热灭活胎牛血清（v/v）或绵羊正常血清可用于降低非特异性结合。此外，可将酸碱度提高至8.5至9.0。
储存条件（工作方案）：必须制备新鲜物料！
工作浓度：共轭物的工作浓度取决于应用和底物。以下浓度应作为指导：糖缀合物中地高辛标记糖的检测：20至50 & # 956；g/ml
- 荧光原位杂交：1-20 & # 956；g/ml
- 免疫组织细胞化学：20-50 & # 956；g/ml
- 原位杂交：20-50 & # 956；g/ml
工作溶液：PBS，0.5%牛血清白蛋白，pH 7.4。
1%封闭剂（w/v）、1-5%热灭活胎牛血清（v/v）或绵羊正常血清可用于降低非特异性结合。此外，可将酸碱度提高至8.5至9.0。
储存条件（工作方案）：必须制备新鲜物料！
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Aquatic Chronic 3 - Skin Sens. 1
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11 - Combustible Solids
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商品
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
地高辛（DIG）标记方法和试剂盒适用于DNA和RNA DIG探针、随机引物DNA标记、切口平移标记、5'和3'寡核苷酸末端标记。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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