生物来源
sheep
质量水平
偶联物
peroxidase conjugate
抗体形式
F(ab′)2 fragment of affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
lyophilized (clear, brown solution after reconstitution)
包装
pkg of 150 U
制造商/商品名称
Roche
储存温度
2-8°C
一般描述
地高辛是一种半抗原，可用于核酸标记和检测系统。本产品含辣根过氧化物酶（POD）标记抗地高辛抗体的Fab片段。抗地高辛-POD Fab片段用于检测地高辛标记的化合物。
特异性
来自羊的多克隆抗体对于地高辛和异羟洋地黄毒苷有特异性，不会和其他类固醇，比如人雌激素和雄激素反应。
应用
使用抗-地高辛-POD，Fab片段用来在以下实验中检测地高辛-标记化合物:
- 斑点印迹法
- ELISA
- 免疫组织化学
- 原位杂交
- Western印迹法
制备说明
在用地高辛免疫后，山羊IgG通过离心交换色谱进行了纯化，而特异性的IgG通过免疫吸附进行了分离。通过木瓜蛋白酶消化获得的Fab片段经过凝胶过滤，和特异性标记偶联，在缓冲液中稳定。
工作浓度: 偶联剂的工作浓度将取决于应用和底物。以下浓度可作为参考：斑点印迹：150 mU/ml
工作溶液：100mM Tris-HCl,150mM NaCl,pH 7.5.
1% 阻断试剂(w/v), 1到5%热失活胎牛血清(v/v)或羊正常血清，可以用来降低非特异性结合。
工作浓度: 偶联剂的工作浓度将取决于应用和底物。以下浓度可作为参考：斑点印迹：150 mU/ml
- ELISA: 50至150 mU/ml
- 免疫组织化学：250至500 mU/ml
- 原位杂交：1.5至7.5 mU/ml
- Southern blot：150 mU/ml
- Western blot：250至500 mU/ml
工作溶液：100mM Tris-HCl,150mM NaCl,pH 7.5.
1% 阻断试剂(w/v), 1到5%热失活胎牛血清(v/v)或羊正常血清，可以用来降低非特异性结合。
重悬
加入1ml双蒸馏水，最终浓度为150 U/ml。
其他说明
仅用于生命科学研究。不可用于诊断。
Not finding the right product?
Try our 产品选型工具.
警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
WGK
WGK 1
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
常规特殊物品
Hande Tunbak et al.
eLife, 9 (2020-05-06)
The zebrafish was used to assess the impact of social isolation on behaviour and brain function. As in humans and other social species, early social deprivation reduced social preference in juvenile zebrafish. Whole-brain functional maps of anti-social isolated (lonely) fish
Yutaka Daido et al.
Developmental biology, 392(1), 117-129 (2014-05-07)
The vertebrate retina contains two types of photoreceptor cells, rods and cones, which use distinct types of opsins and phototransduction proteins. Cones can be further divided into several subtypes with differing wavelength sensitivity and morphology. Although photoreceptor development has been
Sarah E Mercer et al.
PloS one, 7(12), e52375-e52375 (2013-01-10)
The inability to functionally repair tissues that are lost as a consequence of disease or injury remains a significant challenge for regenerative medicine. The molecular and cellular processes involved in complete restoration of tissue architecture and function are expected to
Heather D Bracken-Grissom et al.
Scientific reports, 10(1), 4485-4485 (2020-03-13)
Extraocular photoreception, the ability to detect and respond to light outside of the eye, has not been previously described in deep-sea invertebrates. Here, we investigate photosensitivity in the bioluminescent light organs (photophores) of deep-sea shrimp, an autogenic system in which
Charless C Fowlkes et al.
PLoS genetics, 7(10), e1002346-e1002346 (2011-11-03)
Differences in the level, timing, or location of gene expression can contribute to alternative phenotypes at the molecular and organismal level. Understanding the origins of expression differences is complicated by the fact that organismal morphology and gene regulatory networks could
商品
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
地高辛（DIG）标记方法和试剂盒适用于DNA和RNA DIG探针、随机引物DNA标记、切口平移标记、5'和3'寡核苷酸末端标记。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门