产品名称
抗溴脱氧尿苷, from mouse IgG1 (clone: BMC9318)
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
BMC9318, monoclonal
assay
90% (HPLC and SDS-PAGE)
form
solution
packaging
pkg of 50 μg (500 μl)
manufacturer/tradename
Roche
isotype
IgG1
storage temp.
−20°C
Quality Level
Other Notes
仅用于生命科学研究。不可用于诊断。
Physical form
稳定溶于磷酸盐缓冲盐水，pH 7.4，含有0.09％（w/v）叠氮化钠和0.2％（w/v）明胶。
General description
溴脱氧尿苷抗体是5-溴-2′-脱氧尿苷-5′-单磷酸的单克隆抗体。
Immunogen
表位明显位于DNA螺旋内。在抗体有效结合DNA-BrdU之前，必须将DNA变性为ssDNA。
Analysis Note
溴脱氧尿苷抗体与碘-脱氧尿苷具有10％的交叉反应，但与氟-脱氧尿苷无交叉反应。
与任何内源性胸苷或尿苷均无交叉反应。
尚未测试其与5-Br-UTP的交叉反应，但是有研究人员认为存在交叉反应的可能性很大，因为它们的唯一区别是溴取代的远端核糖上没有羟基。
与任何内源性胸苷或尿苷均无交叉反应。
尚未测试其与5-Br-UTP的交叉反应，但是有研究人员认为存在交叉反应的可能性很大，因为它们的唯一区别是溴取代的远端核糖上没有羟基。
通过以考马斯蓝染色的SDS-PAGE和HPLC检测，得到抗体纯度为90％。
Application
溴脱氧尿苷（Anti-BrdU）抗体适用于监测血液、组织和肿瘤中的增殖细胞，以及使用以下方法检测单细胞水平的BrdU掺入：
- 流式细胞术分析
- 免疫组织细胞化学分析
- 冷冻切片分析
- 石蜡切片分析
Biochem/physiol Actions
该抗体能够与溴脱氧尿苷特异性结合，并与碘尿苷（10％）发生交叉反应。溴脱氧尿苷抗体与氟脱氧尿苷无交叉反应，并且与任何内源性细胞组分（如胸苷或尿苷）无交叉反应。
Features and Benefits
制备：用溴脱氧尿苷-牛血清白蛋白偶联物对BALB/c小鼠进行免疫。将从脾脏中分离的淋巴细胞与Ag8.653骨髓瘤细胞融合，产生BMC 9318克隆。抗体来源于BALB/c小鼠的腹水，并通过离子交换层析进行纯化。
测试次数：250次（流式细胞术）
测试次数：250次（流式细胞术）
Preparation Note
工作浓度：流式细胞术：2 μg/ml (0.2 μg/100 μl/106个细胞)；免疫组织细胞化学分析：6 μg/ml
偶联物的工作浓度取决于应用和底物。应使用含0.1% BSA的PBS（pH 7.4）进行稀释，以维持抗体的稳定性。
偶联物的工作浓度取决于应用和底物。应使用含0.1% BSA的PBS（pH 7.4）进行稀释，以维持抗体的稳定性。
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
No data available
flash_point_c
No data available
法规信息
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