用途
sufficient for 25 labeling reactions
sufficient for 50 blots
质量水平
制造商/商品名称
Roche
环保替代产品特性
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
环保替代产品分类
, Aligned
储存温度
−20°C
一般描述
DIG DNA标记和检测试剂盒是使用便捷的试剂盒，用地高辛-脱氧尿苷三磷酸（dUTP）标记DNA随机引物，通过酶免疫法对杂交产物进行碱解，测定颜色。在此方法中，使用随机寡核苷酸3′-OH末端用作引物，在Klenow聚合酶的作用下合成变性 DNA 的互补 DNA 链。
内容物：
内容物：
- 未标记的对照DNA 1, 100 μg/ml
- 未标记的对照DNA 2, 200 μg/ml
- DNA稀释缓冲液
- DIG标记的对照DNA, 5.2 μg/ml
- 10X 六核苷酸混合物
- 10x dNTP标记混合物
- Klenow 酶，标记等级，2 U/μl
- 抗地高辛-AP结合物，750 U / ml
- NBT/BCIP 浓缩储存液
- 封闭剂
我们致力于为您提供更环保的替代产品，以符合“绿色化学的12项原则”的一项或多项原则要求。本产品为一种安全的化学品。DIG系统是灵敏且具有成本效益的方案，可替代核酸的放射性标记和检测方法。有许多已发表论文证明了DIG系统的通用性，因此，使用放射性标记不再是标记用于杂交的DNA的唯一选择。
特异性
灵敏度和特异性：1 μg模板37℃，1小时培育，标准标记反应会产生260ngDIG-标记DNA，20小时培育会生成780ng。DIG检测灵敏度取决于杂交反应中DIG-标记探针浓度和颜色反应持续时间。
应用
DIG DNA标记和检测试剂盒已用于多种杂交技术：
- Southern 印迹
- Northern 印迹
- 斑点印迹
- 菌落和噬菌斑筛查
包装
1个试剂盒包含10种组分。
制备说明
工作浓度：将抗体稀释到1：5000
其他说明
仅用于生命科学研究。不可用于诊断。
仅试剂盒组分
产品编号
说明
- Unlabeled Control DNA 1 100 µg/ml
- Unlabeled Control DNA 2 200 µg/ml
- DNA Dilution Buffer
- DIG-labeled Control DNA 5.2 µg/ml
- Hexanucleotide Mix 10x concentrated
- dNTP Labeling Mixture 10x concentrated
- Klenow Enzyme, Labeling grade 2 U/µl
- Anti-digoxigenin-AP-conjugate antibody 750 U/ml
- NBT/BCIP Concentrated Stock Solution
- Blocking Reagent
查看所有结果 (10)
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
does not flashNot applicable
闪点(°C)
does not flashNot applicable
法规信息
监管及禁止进口产品
Shun Sawatsubashi et al.
Scientific reports, 8(1), 593-593 (2018-01-14)
CRISPR/Cas9-based genome editing has dramatically accelerated genome engineering. An important aspect of genome engineering is efficient knock-in technology. For improved knock-in efficiency, the non-homologous end joining (NHEJ) repair pathway has been used over the homology-dependent repair pathway, but there remains
Jiaqiang Wang et al.
Cell, 175(7), 1887-1901 (2018-12-15)
In early mammalian embryos, it remains unclear how the first cell fate bias is initially triggered and amplified toward cell fate segregation. Here, we report that a long noncoding RNA, LincGET, is transiently and asymmetrically expressed in the nucleus of
Diana C Garcia-Ramon et al.
Microbial biotechnology, 11(2), 302-316 (2017-10-14)
Bacillus pumilus strain 15.1 was previously found to cause larval mortality in the Med-fly Ceratitis capitata and was shown to produce crystals in association with the spore. As parasporal crystals are well-known as invertebrate-active toxins in entomopathogenic bacteria such as
Nasser Shakhssalim et al.
Cancer cell international, 13(1), 120-120 (2013-12-07)
Bladder cancer is a relatively common and potentially life-threatening neoplasm that ranks ninth in terms of worldwide cancer incidence. The aim of this study was to determine deletions and sequence variations in the mitochondrial displacement loop (D-loop) region from the
Handeng Liu et al.
Journal of invertebrate pathology, 99(2), 235-238 (2008-07-22)
Among Microsporidia, Nosema bombycis has a novel arrangement of LSUrRNA, SSUrRNA, ITS, IGS and 5SrRNA. To determine the distribution of rDNA among the chromosomes, we performed genome-wide screening and Southern blotting with three probes (SSU, ITS and IGS). Southern blotting
商品
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
地高辛（DIG）标记方法和试剂盒适用于DNA和RNA DIG探针、随机引物DNA标记、切口平移标记、5'和3'寡核苷酸末端标记。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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