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一般描述
生物素是一种水溶性维生素，可作为羧化酶的辅酶。生物素-16-dUTP用于产生生物素DNA探针，该探针通常用于不同方法中检测特定的DNA和RNA序列，如Southern和Northern印记，原位杂交和夹心检测。
应用
生物素-16-dUTP 已被用于：
- 非放射性DNA标记 如随机引发、PCR标记或缺口翻译。生物素-16-dUTP 在随机引物 DNA 标记反应或缺口翻译以及PCR中，以 35% 生物素-16-dUTP 和 65% dTTP 的比例取代 dTTP。
- 作为底物 用于 末端转移酶、DNA 聚合酶 I（全酶和 Klenow 片段）、Taq DNA 聚合酶和逆转录酶（如 Transcriptor）。该核苷酸还可作为 3′-末端标记的末端转移酶的底物
- 用于荧光 原位 杂交 (FISH)
生化/生理作用
通过与组蛋白中的赖氨酸残基结合，生物素可影响基因调控和染色质结构。它影响后天免疫T细胞和自然杀伤细胞的活动。小鼠体内生物素缺乏会表现出炎症加重的症状。研究还表明，在生物素缺乏条件下，人类树突状细胞的炎症反应也会加剧。
质量
典型分析：至少85%的脱氧核苷酸三磷酸（生物素-16-dUTP）（高效液相色谱，峰面积百分比）。
注：为了您的方便，生物素-dUTP也可以方便的标记混合物形式提供（传统dNTP和生物素-16-dUTP的单份混合液），同时提供特定技术专用的混合液（一瓶包含核苷酸、缓冲液和酶）。
注：为了您的方便，生物素-dUTP也可以方便的标记混合物形式提供（传统dNTP和生物素-16-dUTP的单份混合液），同时提供特定技术专用的混合液（一瓶包含核苷酸、缓冲液和酶）。
分析说明
吸光度：生物素-16-dUTP的光谱数据(pH 7.3)：
Emax241：E = 10.700;
Emax292: E = 7.000;
Emax265: E = 4.400;
A292/265 : 1.6.
Emax241：E = 10.700;
Emax292: E = 7.000;
Emax265: E = 4.400;
A292/265 : 1.6.
其他说明
生物素标记的DNA可用以下方法检测：链霉亲和碱性磷酸酶偶联物和化学发光底物(CSPD, CDP-Star)或显色底物生物素发光检测试剂盒
储存分类代码
12 - Non Combustible Liquids
WGK
nwg
闪点(°F)
does not flash
闪点(°C)
does not flash
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