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Merck
CN

11089161001

Roche

链霉抗生物素蛋白-AP缀合物

solution, pkg of 1 mL (1,000U)

别名:

链霉亲和素

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About This Item

UNSPSC代码:
12352200

形式

solution

质量水平

包装

pkg of 1 mL (1,000U)

制造商/商品名称

Roche

储存温度

2-8°C

一般描述

链霉亲和素与碱性磷酸酶 (AP) 共价偶联,用于制备链霉亲和素-AP 偶联物。

碱性磷酸酶
碱性磷酸酶常被用作酶免疫测定的标记物。AP 将敏感底物 X-磷酸盐/NBT 转化为稳定的水溶性产物。

应用

链霉亲和素-AP 已用于检测生物素化化合物,用于:
  • ELISA
  • 免疫组织化学
  • 蛋白印迹

外形

溶液,在 50 mM 三乙醇胺缓冲液、3 MNaCl、1 mM MgCl 2 、0.1 mM ZnCl 2 、10 mg/mL 牛血清白蛋白 (pH 7.6) 中稳定

制备说明

工作溶液: 50-1,000 mU/ml(即贮备液可稀释)。将缀合物储备溶液在 100 mM Tris-HCl、150 mM NaCl (pH 7.5) 中稀释。

工作浓度: 缀合物的工作浓度取决于应用和底物。标示浓度应作为指导。

ELISA:50-200 mU/ml(1:5000-1:20,000 稀释)
免疫组织化学/细胞化学:250-1,000 mU/ml(1:1000-1:4000 稀释)
免疫印迹:200-500 mU/ml(1:2000-1:5000 稀释)

稳定性: 浓缩储备液在 2 ~ 8°C 下可稳定储存至标签上打印的有效期为止。 切勿冻结!

其他说明

仅用于生命科学研究。不可用于诊断。

相关产品

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警示用语:

Warning

危险声明

危险分类

Skin Sens. 1

WGK

WGK 1

闪点(°F)

No data available

闪点(°C)

No data available

法规信息

常规特殊物品
含少量动物源组分生物产品

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Ablation of BMP signaling hampers the blastema formation in Poecilia latipinna by dysregulating the extracellular matrix remodeling and cell cycle turnover
Patel S, et al.
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Manuela Sauter et al.
STAR protocols, 3(3), 101664-101664 (2022-09-14)
Different types of immune cells are involved in atherogenesis and may act atheroprotective or atheroprogressive. Here, we describe an in vitro approach to analyze CD11c+ cells and CD11c+-derived ApoE in atherosclerosis. The major steps include harvesting mouse bone marrow, plating
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Molecular genetics and metabolism, 95(4), 201-205 (2008-11-01)
We investigated in a patient with holocarboxylase synthetase deficiency, the relation between the biochemical and genetic factors of the mutant protein with the pharmacokinetic factors of successful biotin treatment. A girl exhibited abnormal skin at birth, and developed in the
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iScience, 25(1), 103677-103677 (2022-01-18)
Atherosclerosis is studied in models with dysfunctional lipid homeostasis-predominantly the ApoE-/- mouse. The role of antigen-presenting cells (APCs) for lipid homeostasis is not clear. Using a LacZ reporter mouse, we showed that CD11c+ cells were enriched in aortae of ApoE-/-
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Bone morphogenetic proteins play a pivotal role in the epimorphic regeneration in vertebrates. Blastema formation is central to the epimorphic regeneration and crucially determines its fate. Despite an elaborate understanding of importance of Bone morphogenetic protein signaling in regeneration, its

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