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一般描述
链霉亲和素与辣根过氧化物酶共价结合。结合物可用于检测生物素标记的物质。
应用
链霉亲和素-过氧化物酶(POD)结合物已被用于：
- ELISA
- 原位杂交
- 蛋白质免疫印迹分析
单位定义
一单位链霉亲和素-POD结合物，相当于一单位过氧化物酶，将在+ 25°C和pH 7.0下在1分钟内氧化1 μmol愈创木酚四聚体。
单位换算：1U（+ 25°C；愈创木酚作为底物；pH7.0）对应于1.24 U（+ 25°C；1.7 mM ABTS作为底物；pH = 6.0）或4.0 U（+ 25°C；8.7 mM ABTS作为底物；pH = 5.0）。
单位换算：1U（+ 25°C；愈创木酚作为底物；pH7.0）对应于1.24 U（+ 25°C；1.7 mM ABTS作为底物；pH = 6.0）或4.0 U（+ 25°C；8.7 mM ABTS作为底物；pH = 5.0）。
外形
液体，稳定化的
制备说明
工作浓度：5 - 500 mU/ml （即储备溶液可稀释）。偶联物的工作浓度取决于应用和底物。以下浓度应作为使用指南：
- ELISA：50至200 mU/ml
- 免疫组织化学：250至1000mU/ml
- 蛋白质印迹：200-250 mU/ml
其他说明
仅用于生命科学研究。不可用于诊断。
警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
常规特殊物品
含少量动物源组分生物产品
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