一般描述
链霉亲和素与辣根过氧化物酶共价结合。结合物可用于检测生物素标记的物质。
应用
链霉亲和素-过氧化物酶(POD)结合物已被用于：
- ELISA
- 原位杂交
- 蛋白质免疫印迹分析
单位定义
一单位链霉亲和素-POD结合物，相当于一单位过氧化物酶，将在+ 25°C和pH 7.0下在1分钟内氧化1 μmol愈创木酚四聚体。
单位换算：1U（+ 25°C；愈创木酚作为底物；pH7.0）对应于1.24 U（+ 25°C；1.7 mM ABTS作为底物；pH = 6.0）或4.0 U（+ 25°C；8.7 mM ABTS作为底物；pH = 5.0）。
单位换算：1U（+ 25°C；愈创木酚作为底物；pH7.0）对应于1.24 U（+ 25°C；1.7 mM ABTS作为底物；pH = 6.0）或4.0 U（+ 25°C；8.7 mM ABTS作为底物；pH = 5.0）。
外形
液体，稳定化的
制备说明
工作浓度：5 - 500 mU/ml （即储备溶液可稀释）。偶联物的工作浓度取决于应用和底物。以下浓度应作为使用指南：
- ELISA：50至200 mU/ml
- 免疫组织化学：250至1000mU/ml
- 蛋白质印迹：200-250 mU/ml
其他说明
仅用于生命科学研究。不可用于诊断。
警示用语：
Warning
危险声明
危险分类
Skin Sens. 1
WGK
WGK 1
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
常规特殊物品
含少量动物源组分生物产品
Husheem Michael et al.
Frontiers in immunology, 11, 196-196 (2020-03-03)
Human rotavirus (HRV) is a leading cause of morbidity and mortality in children, especially in developing countries. Malnutrition is prevalent in these countries, which may contribute to the decreased oral vaccine efficacy, posing a concern for global health. Neonatal gnotobiotic
Patrick Neckermann et al.
Frontiers in immunology, 12, 761214-761214 (2021-11-16)
Persistent human papillomavirus (HPV) infections are causative for cervical neoplasia and carcinomas. Despite the availability of prophylactic vaccines, morbidity and mortality induced by HPV are still too high. Thus, an efficient therapy, such as a therapeutic vaccine, is urgently required.
Benjamin Meyer et al.
Frontiers in immunology, 13, 841009-841009 (2022-05-20)
To comprehensively evaluate SARS-CoV-2 specific B-cell and antibody responses up to one year after mild COVID-19. In 31 mildly symptomatic COVID-19 participants SARS-CoV-2-specific plasmablasts and antigen-specific memory B cells were measured by ELISpot. Binding antibodies directed against the proteins spike
BAP1 and PTEN mutations shape the immunological landscape of clear cell renal cell carcinoma and reveal the intertumoral heterogeneity of T cell suppression: a proof-of-concept study.
Friedhoff, et al.
Cancer Immunology, Immunotherapy, 72, 1603-1618 (2023)
Angelika Hammerer-Lercher et al.
Clinical chemistry, 54(5), 858-865 (2008-03-15)
The specific forms of pro-B-type natriuretic peptide (proBNP) that occur in human blood are not yet clear. We demonstrated the presence of several proBNP forms in human plasma with a new affinity chromatography method that can be used in combination
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门