生物来源
human serum (transferrin)
synthetic (sodium selenite)
质量水平
重组
expressed in human cells
无菌性
non-sterile; 0.2 μm filtered
检测方案
>98% (Transferrin, SDS-PAGE)
>99% (Insulin, HPLC)
>99% (Sodium selenite)
形式
lyophilized
包装
pkg of 50 mg (for 5 l medium)
制造商/商品名称
Roche
技术
cell culture | mammalian: suitable
溶解性
water: miscible
储存温度
2-8°C
基因信息
bovine ... INS(280829)
human ... TFRC(7037)
一般描述
胰岛素-转铁蛋白-亚硒酸钠(ITS)添加剂对于很多细胞类型来说都是最为重要的3种生长因子。胰岛素是所有原代细胞和细胞系的无血清培养基配方成分。在无血清培养基中用于促进细胞生长、增加脂肪酸和糖原合成。转铁蛋白是许多类型细胞必需的生长因子。硒常用于细胞生长优化。ITS可作为补偿，代替相当可观的细胞生长所需的血清用量。ITS也可作为抗氧化剂，支持体内生产(IVD)胚胎的生长。其中，胰岛素可促进胚胎生长和代谢。转铁蛋白介导胚胎中的铁转运。亚硒酸钠保护细胞免于氧化损伤并抑制脂质过氧化。ITS可用于人软骨细胞单层培养。
胰岛素-转铁蛋白-亚硒酸钠补充剂以最佳比例包含无血清培养基中最重要的促生长成分。
来源：胰岛素-牛胰腺；转铁蛋白-人血清；合成亚硒酸钠
来源：胰岛素-牛胰腺；转铁蛋白-人血清；合成亚硒酸钠
应用
胰岛素-转铁蛋白-亚硒酸钠补充剂在细胞培养培养基中用作基础生长因子补充剂，向其中添加其他细胞类型特异性营养素和生长因子。
制备说明
工作浓度：5 μg/mL胰岛素、5 μg/mL转铁蛋白、5 ng/mL亚硒酸钠（3.0 x 10-8 M）
储存条件（工作溶液）：-15至-25℃。
制备适当的等分试样并避免反复冻融。
储存条件（工作溶液）：-15至-25℃。
制备适当的等分试样并避免反复冻融。
重悬
胰岛素-转铁蛋白-亚硒酸钠补充剂应在5 ml或25 ml无菌双蒸水（1000 x 浓缩储备液）中复溶。
。用培养基进一步稀释。
。用培养基进一步稀释。
其他说明
仅用于生命科学研究。不用于诊断操作。
WGK
WGK 1
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
常规特殊物品
K H Chua et al.
European cells & materials, 9, 58-67 (2005-06-18)
This study was to investigate the effects of insulin-transferrin-selenium (ITS) on the proliferation and quantitative gene expression of adult human nasal septum chondrocytes in monolayer culture expansion and the formation of tissue engineered hyaline cartilage. Effects of ITS on human
Imran Khan et al.
Theriogenology, 152, 147-155 (2020-05-16)
Hypothermic storage of gametes and embryos at 4 °C can be used as an alternative to cryopreservation, but hypothermic preservation can maintain embryo viability for a short duration only. This study investigated the effect of insulin-transferrin-sodium selenite (ITS) in embryo culture
Jyoti Motwani et al.
Epigenomics, 13(8), 577-598 (2021-03-31)
Aims & objectives: The aim of this study was to investigate the role of DNA methylation in invasiveness in melanoma cells. Materials & methods: The authors carried out genome-wide transcriptome (RNA sequencing) and reduced representation bisulfite sequencing methylome profiling between noninvasive (n = 4)
María Virumbrales-Muñoz et al.
Lab on a chip, 20(23), 4420-4432 (2020-10-27)
Clear cell renal cell carcinoma (ccRCC) is a common genitourinary cancer associated with the development of abnormal tumor angiogenesis. Although multiple anti-angiogenic therapies have been developed, responses to individual treatment are highly variable between patients. Thus, the use of one-patient
Milu T Cherian et al.
The Journal of biological chemistry, 287(28), 23368-23380 (2012-05-17)
The androgen receptor (AR) has a critical role in the growth and progression of androgen-dependent and castration-resistant prostate cancers. To identify novel inhibitors of AR transactivation that block growth of prostate cancer cells, a luciferase-based high-throughput screen of ~160,000 small
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