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表单
lyophilized
质量水平
用途
sufficient for ≤400 reactions
包装
pkg of 2 mg (Primer, Random pd(N)6 Potassium Salt)
制造商/商品名称
Roche
储存温度
−20°C
一般描述
p(dN)6
应用
cDNA合成引物
随机引物已用于：
- 逆转录聚合酶链式反应(RT-PCR)和定量PCR(qPCR)逆转录RNA
- 将RNA样本逆转录为cDNA
- 微滴数字PCR(ddPCR)法以RNA合成cDNA
特点和优势
- 对mRNA中的所有RNA序列具有一致的代表性
- 可用于引发无poly(A)尾RNA的cDNA转录
- 短cDNA转录本可克服RNA二级结构带来的难题
- 可控制cDNA的平均长度，只要调节逆转录(RT)反应中随机引物与RNA的比例
其他说明
仅用于生命科学研究。不可用于诊断。
储存分类代码
12 - Non Combustible Liquids
WGK
nwg
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
常规特殊物品
Monika Hiller et al.
PloS one, 13(10), e0204485-e0204485 (2018-10-03)
Duchenne muscular dystrophy is a lethal disease caused by lack of dystrophin. Skipping of exons adjacent to out-of-frame deletions has proven to restore dystrophin expression in Duchenne patients. Exon 51 has been the most studied target in both preclinical and
Vadim Alexandrov et al.
Nature biotechnology, 34(8), 838-844 (2016-07-05)
Rapid technological advances for the frequent monitoring of health parameters have raised the intriguing possibility that an individual's genotype could be predicted from phenotypic data alone. Here we used a machine learning approach to analyze the phenotypic effects of polymorphic
Amaresh C Panda et al.
Nucleic acids research, 44(5), 2393-2408 (2016-01-29)
Skeletal muscle contains long multinucleated and contractile structures known as muscle fibers, which arise from the fusion of myoblasts into multinucleated myotubes during myogenesis. The myogenic regulatory factor (MRF) MYF5 is the earliest to be expressed during myogenesis and functions
Vittoria Pagliarini et al.
Journal of neurochemistry, 153(2), 264-275 (2019-12-08)
Spinal muscular atrophy (SMA) is a motor neuron disease caused by loss of function mutations in the Survival Motor Neuron 1 (SMN1) gene and reduced expression of the SMN protein, leading to spinal motor neuron death, muscle weakness and atrophy.
Piergiorgio La Rosa et al.
Journal of neurochemistry, 157(4), 1153-1166 (2020-09-23)
Neural Progenitor Cells (NPCs) are multipotent cells that are able to self-renew and differentiate into neurons. The size of the initial pool of NPCs during the brain development strongly affects the number of neurons that compose cortical multi-layer during development.
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