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Merck
CN

10838039001

Roche

纤连蛋白

from human plasma

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别名:
纤连蛋白 溶液 来源于人类成纤维细胞
MDL编号:
UNSPSC代码:
12352207

生物来源

human plasma

质量水平

无菌性

non-sterile; 0.2 μm filtered

检测方案

95% (SDS-PAGE)

形式

lyophilized (clear, colorless solution after reconstitution)

分子量

440 kDa

包装

pkg of 5 mg

制造商/商品名称

Roche

储存条件

avoid repeated freeze/thaw cycles

技术

cell culture | mammalian: suitable

UniProt登记号

储存温度

2-8°C

基因信息

human ... FN1(2335)

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一般描述

纤连蛋白负责细胞与细胞外基质的相互作用。参与细胞的黏附、迁移、生长和分化。是血浆和其他体液的可溶性成分。此外,它也是不溶性细胞外基质的一部分。

特异性

对大多数哺乳动物细胞有活性

应用

纤维连接蛋白促进许多细胞的附着和随后的扩散。用于培养皿的包被。

测试方法

促进 3T3 (NR6) 细胞粘附的检测(XTT 切割)

外形

在冻干过滤通过 0.2μm 孔径的膜之前,冷冻干燥物(每 mg 纤连蛋白含有 1.126mg 甘氨酸和 0.058mg 氯化钠)。

制备说明

工作浓度: 5μg/cm 2
5μg/cm 2 对于细胞培养容器的包衣
储存条件(工作液):-15°C 至-25°C
复溶溶液在-15°C 至-25°C 下稳定。
注意: 将复溶溶液分等份储存于-15 至-25°C 条件下。
避免反复冻融。

重悬

分别加入 1 或 5 mL 无菌水,得到最终浓度为 1 mg/mL 的溶液。在 37°C 下孵育 30 至 60 min 以溶解。 不要搅拌。
复溶后,溶液可能含有少量不溶性聚集物。这些聚集体是纤连蛋白固有的现象,不影响产品性能。

其他说明

仅用于生命科学研究。不可用于诊断。

免责声明

仅供研究使用。在法国,在用于科研目的时(包括进口和出口活动(《公共健康法》第L 1211-1条,第2节))该产品为管制品。购买者(即最终用户)需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。

WGK

WGK 1

闪点(°F)

does not flash

闪点(°C)

does not flash

法规信息

常规特殊物品

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