DNA Molecular Weight Marker V

Fragment mixture prepared by cleavage of pBR322 DNA with restriction endonuclease Hae III.

Size Range: 8 to 587 bp

DNA Molecular Weight Marker V is used for the size determination of DNA in agarose gels. It simplifies accurate molecular weight determination of double-stranded DNA fragments generated by:

<UL><LI>Restriction digests</LI>

<LI>PCR</LI>

<LI>RT-PCR</LI></UL>

Ready-to-use solution, 250μg/ml, in TE buffer (10mM Tris-HCl, 1mM EDTA, pH 8.0)

The mixture contains 22 DNA fragments with the following base pair lengths (1 base pair = 660 daltons): 8, 11, 18, 21, 51, 57, 64, 80, 89, 104, 123, 124, 184, 192, 213, 234, 267, 434, 458, 504, 540, and 587 as determined by computer analysis of the pBR322 sequence.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

50 μg = 1A₂₆₀ unit

Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents

Working concentration: A suggested working ratio, for immediate use in coupling procedures, is 2 mg POD to 4 mg lgG (molar ratio = 2:1, POD:IgG).

Storage conditions (working solution): 2 to 8 °C
Once thawed we recommend further storage at 2 to 8 °C.

Store at -15–-25 °C. (until use)

仅用于生命科学研究。不可用于诊断。