推荐产品
形式
lyophilized (salt-free)
比活
20 U/mg (Approximately 20 U/mg lyophilizate at +25°C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at +37°C with casein as the substrate).)
分子量
30 kDa
包装
pkg of 2 mg
制造商/商品名称
Roche
最佳pH
8.0-8.5
一般描述
内切蛋白酶Glu-C是一种葡萄球菌丝氨酸蛋白酶。其抑制剂包括DFP、α2-巨球蛋白、以及TLCK。
At 25 °C with Z-Phe-Leu-Glu-4-nitranilide as the substrate (approximately 500 U/mg lyophilizate at 37 °C with casein as the substrate).
特异性
热灭活：通过煮沸10分钟使内切蛋白酶Glu-C失活。
应用
使用内切蛋白酶Glu-C（V8蛋白酶）进行蛋白结构分析和序列分析。
生化/生理作用
内切蛋白酶Glu-C可特异性地水解Glu（或同时水解Glu和Asp）羧基侧的肽和酯键，其取决于所用的缓冲液。
制备说明
激活剂：该酶在SH试剂存在下具有最大活性
工作浓度：1 至 5 mM
工作溶液: 推荐的溶剂是50mM乙酸铵（pH 4.0，2mg/ml）。
储存条件（工作溶液）：-15至-25°C
分液冷冻保存并仅解冻一次，酶（2mg/ml，溶于50mM乙酸铵中，pH 4.0）可稳定保存至少一个月。
工作浓度：1 至 5 mM
工作溶液: 推荐的溶剂是50mM乙酸铵（pH 4.0，2mg/ml）。
储存条件（工作溶液）：-15至-25°C
分液冷冻保存并仅解冻一次，酶（2mg/ml，溶于50mM乙酸铵中，pH 4.0）可稳定保存至少一个月。
储存及稳定性
在2°C至8°条件下储存。 （请保持干燥！）
其他说明
仅用于生命科学研究。不可用于诊断。
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 1
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
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