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Merck
CN

10775835001

Roche

牛血清白蛋白

98.5% (electrophoresis), Fatty acids (total) ≤0.2 mg/g, Triglycerides (enzym.) free, Immunoglobulins not detectable, microbiological culture: suitable, USA origin

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别名:
BSA, Bovine Serum Albumin Fraction V, fatty acid free, Bovine albumin, bovine serum
UNSPSC代码:
12352106

生物来源

bovine

质量水平

检测方案

98.5% (electrophoresis)

形式

lyophilized

分子量

Mr 68 kDa

包装

pkg of 50 g

制造商/商品名称

Roche

来源

USA origin

技术

immunofluorescence: suitable
ligand binding assay: suitable

杂质

Immunoglobulins, none detected
Triglycerides, none detected
≤0.2 mg/g fatty acids

UniProt登记号

运输

wet ice

储存温度

2-8°C

基因信息

bovine ... ALB(280717)

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一般描述

Highest quality for fatty acid and lipid research.
BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7, it contains 17 intra-chain disulfide bridges and one sulfhydryl group.

应用

Bovine Serum Albumin, Fraction V, fatty acid free is used:
  • in lipid research to study lipid metabolism in biological systems
  • to identify transport mechanisms in living organisms
  • to study ligand binding
  • to investigate ionic binding
  • in the preparation of solutions during isolation of cardiomyocytes
  • as a blocking solution to incubate embryos for immunofluorescence
  • in dual polarisation interferometry (DPI) to study the interaction between membrane active peptides and artificial bilayers

生化/生理作用

The fatty acids that are insoluble in circulating plasma are transported primarily by serum albumin. The human serum albumin and bovine serum albumin share 76% sequence homology. Being the most abundant protein in the circulatory system, it participates in the maintenance of blood pH and carries several endogenous and exogenous compounds, including fatty acids, metal, amino acids, steroids and drugs.

特点和优势

  • Free from fatty acids according to current quality control procedures (suitable for lipid research)
  • High quality
  • Low content of heavy metal ions
  • Long-term stability

质量

Purity: ≥98.5 % albumin (electrophoresis), 98% protein
Contaminants: <0.2 mg fatty acids/g, triglycerides not detectable (enzymatic), immunoglobulins not detectable (Ouchterlony), ≤5% H2O,≤30 ppm heavy metals

其他说明

仅用于生命科学研究。不可用于诊断。

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

监管及禁止进口产品

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Examination of the Interaction between a Membrane Active Peptide
and Artificial Bilayers by Dual Polarisation Interferometry
Payne JAE, et al.
Bio-protocol, 7(1), 1-12 (2017)
Putnam F
The Plasma Proteins: Structure, Function, and Genetic Control, 5 (2012)
Haneen Kayyal et al.
eLife, 10 (2021-07-06)
To survive in an ever-changing environment, animals must detect and learn salient information. The anterior insular cortex (aIC) and medial prefrontal cortex (mPFC) are heavily implicated in salience and novelty processing, and specifically, the processing of taste sensory information. Here
Jelena Branovets et al.
American journal of physiology. Heart and circulatory physiology, 305(4), H506-H520 (2013-06-25)
Disruption of the creatine kinase (CK) system in hearts of CK-deficient mice leads to changes in the ultrastructure and regulation of mitochondrial respiration. We expected to see similar changes in creatine-deficient mice, which lack the enzyme guanidinoacetate methyltransferase (GAMT) to
Geronimo Matteo et al.
Endocrinology, 162(6) (2021-03-12)
Human studies consistently show an association between exposure to persistent organic pollutants, including 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD, aka "dioxin"), and increased diabetes risk. We previously showed that a single high-dose TCDD exposure (20 µg/kg) decreased plasma insulin levels in male and female

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