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主要文件

安全信息

10745782001

Roche

DNA,λ

from bacteriophage lambda cl857 Sam 7

别名:

λdna

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1 ML
CN¥1,763.72

CN¥1,763.72


预计发货时间April 27, 2025详情



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变更视图
1 ML
CN¥1,763.72

About This Item

UNSPSC代码:
41106310

CN¥1,763.72


预计发货时间April 27, 2025详情


质量水平

表单

solution

分子量

32,300 kDa

包装

pkg of 1 mL

制造商/商品名称

Roche

储存条件

avoid repeated freeze/thaw cycles

浓度

250 μg/mL

杂质

RNA, none detected
nuclease, none detected

颜色

colorless

溶解性

water: miscible

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1 of 4

此商品
D9281D2916P9614
Quality Level

100

Quality Level

100, 200

Quality Level

100, 200

Quality Level

200

manufacturer/tradename

Roche

manufacturer/tradename

-

manufacturer/tradename

-

manufacturer/tradename

-

form

solution

form

liquid

form

buffered aqueous solution

form

liquid

color

colorless

color

-

color

-

color

-

packaging

pkg of 1 mL

packaging

-

packaging

-

packaging

-

concentration

250 μg/mL

concentration

-

concentration

-

concentration

≥400000 units/mL

一般描述

噬菌体 λ 的 DNA 含有 48,502 个核苷酸对,形成线性双链。这种 DNA 分子的 5′ 端有 12 个核苷酸的自互补单链突起,可退火形成圆圈。进入宿主细胞后,DNA 在 DNA 连接酶存在下环化。许多这样的圆圈是在溶菌感染早期产生的。在复制的后期,这些单独的环/环被称为滚环复制的过程转化成线性连通体。线性连体在包装过程中被酶 DNA 终止酶切割形成单体染色体单位。[1]

应用

λDNA(DNA, lambda)已用于在基因组DNA分离前注入外周血白细胞(PBL)。[2]它也用于制备生物素化双链DNA(dsDNA)底物。[3]

质量

限制性核酸内切酶 EcoR I 和 Hind III 酶切后的 DNA 在凝胶电泳中显示野生型 λDNA 的典型酶切图谱。

序列

链长 48,502 bp

外形

溶液,250μg/ml,10 mM Tris-HCl,1 mM EDTA,pH 8.0,即用型

储存及稳定性

使用前储存于-15至-25°C。解冻后,储存于2-8°C。

其他说明

仅用于生命科学研究。不可用于诊断。

储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

No data available

闪点(°C)

No data available

法规信息

常规特殊物品

  • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number

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    访问文档库

    Bacteriophage lambda DNA: the beginning of the end.
    A Becker et al.
    Journal of bacteriology, 172(6), 2819-2824 (1990-06-01)
    Isolation of high quality DNA and RNA from leaves of the carnivorous plant Drosera rotundifolia.
    Bekesiova I, et al.
    Plant Molecular Biology Reporter, 17, 269-277 (1999)
    Rachel Howard-Till et al.
    BioTechniques, 70(2), 120-125 (2020-12-15)
    Genetic characterization of wild and cultivated plants provides valuable knowledge for conservation and agriculture. DNA sequencing technologies are improving, and costs are dropping. Yet analysis of many species is hindered because they grow in regions that lack infrastructure for advanced
    Shaun Wynyard et al.
    Journal of virological methods, 175(1), 95-100 (2011-05-07)
    Porcine Endogenous Retrovirus (PERV) poses an infectious risk in the field of xenotransplantation. This risk may be mitigated by breeding selectively animals bearing favorable PERV genetic characteristics including pigs with low levels of PERV integrated in the genome. A real-time
    Darren Yang et al.
    Nature communications, 7, 11026-11026 (2016-03-18)
    We present a miniature centrifuge force microscope (CFM) that repurposes a benchtop centrifuge for high-throughput single-molecule experiments with high-resolution particle tracking, a large force range, temperature control and simple push-button operation. Incorporating DNA nanoswitches to enable repeated interrogation by force

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