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安全信息

10602442001

Roche

α2-Macroglobulin

from bovine plasma

别名:

universal protease inhibitor

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About This Item

UNSPSC代码:
12352204

生物来源

bovine plasma

表单

lyophilized (Contains 6% BSA)

包装

pkg of 25 IU

制造商/商品名称

Roche

技术

blocking: suitable

pH值范围

4-7

UniProt登记号

运输

wet ice

基因信息

bovine ... A2M(513856)

一般描述

α2-Macroglobulin belongs to the macroglobulin superfamily. It is a high molecular weight tetrameric glycoprotein with receptor-binding domain (RBD), thioester-containing domain (TED) domain and C1r/C1s, Uegf, Bmp1 (CUB) domain.

应用

α2-Macroglobulin has been used as a component of homogenization buffer for processing hemibrains of young mice.

生化/生理作用

α2-Macroglobulin (α2M) binds several hormones and may regulate their activity. α2M also aids protection against various infections.
α2-Macroglobulin is a plasma endopeptidase and glycoprotein which functions on proteinases, especially serine proteases. It interacts with and inhibits endopeptidases. However, it does not act on other proteases, including inactive proteinases.

序列

α2-macroglobulin, a glycoprotein, is a tetramer of identical subunits, which are Iinked in pairs by disulfide bonds.

制备说明

Working solution: Recommended solvent is distilled water.
Storage conditions (working solution): -15 to -25 °C

重悬

Soluble in water. Stable for at least one week at room temperature (15 to 25 °C), or 3 weeks at 2 to 8 °C. Can also be frozen in aliquots at -15 to -25 °C, where it remains stable for at least 6 months. Sensitive to acidic pH, denatured below pH 4.0. Ammonia methylamine and hydroxylamine (above pH 7.0) cause irreversible conversion to the inactive form.
Note: Do not use α2-macroglobulin in the presence of DTT since it causes a (reversible) dissociation into inactive subunits. α2-macroglobulin acts by physically entrapping endoproteinases, usually in a 1:1 ratio.

储存及稳定性

Store at 2 to 8 °C. (Store dry!)

分析说明

Specific activity: approximately 1 inhibitor U/mg protein (tested with trypsin at +25°C and Chromozym TRY as substrate).
Crossreactivity with pregnancy associated glycoproteins: deglycosylation of α2-macroglobulin is recommended.

其他说明

仅用于生命科学研究。不可用于诊断。

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

does not flash

闪点(°C)

does not flash

法规信息

监管及禁止进口产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Ahmed A Rehman et al.
Journal of cellular physiology, 228(8), 1665-1675 (2012-10-23)
Alpha macroglobulins are large glycoproteins which are present in the body fluids of both invertebrates and vertebrates. Alpha-2-macroglobulin (α2 M), a key member of alpha macroglobulin superfamily, is a high-molecular weight homotetrameric glycoprotein. α2 M has many diversified and complex
A J Barrett et al.
The Biochemical journal, 181(2), 401-418 (1979-08-01)
alpha 2-Macroglobulin (alpha 2M) was isolated from human plasma by a four-step procedure: poly(ethylene glyco) fractionation, gel chromatography, euglobulin precipitation and immunoadsorption. No contaminants were detected in the final preparations by electrophoresis or immunoprecipitation. The protein ran as a single
Fan Yang et al.
Journal of cellular physiology, 232(8), 2253-2262 (2016-12-21)
Porcine induced pluripotent stem cells (piPSCs) retain the enormous potential for farm animal reproduction and translational medicine, and have been reported by many laboratories worldwide. Some piPSC lines were bFGF-dependence and showed mouse EpiSC-like morphology; other lines were LIF-dependence and
The interaction of α2-macroglobulin with proteinases. Characteristics and specificity of the reaction, and a hypothesis concerning its molecular mechanism.
Barrett A J and Phyllis M S
The Biochemical Journal, 133(4), 709-724 (1973)
Matthew R Barron et al.
Frontiers in immunology, 11, 293-293 (2020-03-21)
Inflammation is considered a mechanistic driver of Alzheimer's disease, thought to increase tau phosphorylation, the first step to the formation of neurofibrillary tangles (NFTs). To further understand how inflammation impacts the development of tau pathology, we used (hTau) mice, which

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