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表单
lyophilized
分子量
330 Da
包装
pkg of 5 U
制造商/商品名称
Roche
吸光度比值
A290/260 nm 0.03-0.12
A280/260 nm 0.28-0.39
A250/260 nm 0.84-0.94
储存温度
−20°C (−15°C to −25°C)
一般描述
Poly(dA) is a polymer of polydeoxyadenylic acid and takes up a single stranded structure. It is susceptible to UV irradiation resulting in photoreaction due to the presence of adjacent deoxyadenylic acid units. Poly(dA) along with β-glucan is used for the conjugation and delivery of DNA into cell lines.
应用
Poly(dA) is suitable for use in:
- DNA polymerase assay and as a buffer component for the dilution of protein DNA complex for electrophoretic mobility shift assay (EMSA) studies
- as ssDNA substrate for DNA helicase gene 40 product (G40P) NTP hydrolysis
- in Pyrococcus abyssi DNA polymerase assay
序列
Chain Length Mean chain length lot 20-17: 250-500 bases.
单位定义
1 A260 unit corresponds to 40 μg ssDNA.
其他说明
仅用于生命科学研究。不可用于诊断。
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
The hyperthermophilic euryarchaeota Pyrococcus abyssi likely requires the two DNA polymerases D and B for DNA replication
Journal of molecular biology, 350(1), 53-64 (2005)
Human DNA polymerase lambda functionally and physically interacts with proliferating cell nuclear antigen in normal and translesion DNA synthesis
The Journal of biological chemistry, 277(50), 48434-48440 (2002)
The EMBO journal, 14(11), 2570-2579 (1995-06-01)
Many yeast promoters contain homopolymeric dA:dT sequences that affect nucleosome formation in vitro and are required for wild-type levels of transcription in vivo. Here, we show that poly(dA:dT) is a novel promoter element whose function depends on its intrinsic structure
A novel gene carrier prepared from triple helical beta-glucan and polydeoxyadenylic acid
Journal of materials chemistry. B, 2(8), 933-944 (2014)
A specific photoreaction in polydeoxyadenylic acid
Proceedings of the National Academy of Sciences of the USA, 70(9), 2683-2686 (1973)
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