form
lyophilized
mol wt
330 Da
packaging
pkg of 5 U
manufacturer/tradename
Roche
absorbance ratio
A290/260 nm 0.03-0.12, A280/260 nm 0.28-0.39, A250/260 nm 0.84-0.94
storage temp.
−20°C (−15°C to −25°C)
General description
Poly(dA) is a polymer of polydeoxyadenylic acid and takes up a single stranded structure. It is susceptible to UV irradiation resulting in photoreaction due to the presence of adjacent deoxyadenylic acid units. Poly(dA) along with β-glucan is used for the conjugation and delivery of DNA into cell lines.
Application
Poly(dA) is suitable for use in:
- DNA polymerase assay and as a buffer component for the dilution of protein DNA complex for electrophoretic mobility shift assay (EMSA) studies
- as ssDNA substrate for DNA helicase gene 40 product (G40P) NTP hydrolysis
- in Pyrococcus abyssi DNA polymerase assay
Other Notes
1 A260 unit corresponds to 40 μg ssDNA.
Chain Length Mean chain length lot 20-17: 250-500 bases.
For life science research only. Not for use in diagnostic procedures.
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
V Iyer et al.
The EMBO journal, 14(11), 2570-2579 (1995-06-01)
Many yeast promoters contain homopolymeric dA:dT sequences that affect nucleosome formation in vitro and are required for wild-type levels of transcription in vivo. Here, we show that poly(dA:dT) is a novel promoter element whose function depends on its intrinsic structure
Human DNA polymerase lambda functionally and physically interacts with proliferating cell nuclear antigen in normal and translesion DNA synthesis
Maga G, et al.
The Journal of biological chemistry, 277(50), 48434-48440 (2002)
A specific photoreaction in polydeoxyadenylic acid
Porschke D
Proceedings of the National Academy of Sciences of the USA, 70(9), 2683-2686 (1973)
A novel gene carrier prepared from triple helical beta-glucan and polydeoxyadenylic acid
Liu Q, et al.
Journal of materials chemistry. B, 2(8), 933-944 (2014)
The hyperthermophilic euryarchaeota Pyrococcus abyssi likely requires the two DNA polymerases D and B for DNA replication
Henneke G, et al.
Journal of molecular biology, 350(1), 53-64 (2005)
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