产品名称
GTP-γ-S, Tetralithium salt
color
white
description
C10H12N5O13P3SLi4
assay
87% (HPLC)
form
solid
mol wt
Mr 539.2 (GTP-γ-S)
Mr 563.0 (GTP-γ-S-Li4)
composition
GDP, ≤12% HPLC
GMP, ≤1% HPLC
GTP, ≤2% HPLC
packaging
pkg of 10 mg
manufacturer/tradename
Roche
storage condition
protect from light
concentration
97%
technique(s)
activity assay: suitable
solubility
water: soluble
storage temp.
−20°C (−15°C to −25°C)
Quality Level
Analysis Note
吸光度: 在水溶液中采用254nm吸光法测定含量。
条件：
条件：
- 约10 mg/ml双蒸水。
- 稀释度：9.8 ml双蒸水 + 0.2 ml样品。
Application
GTP-γ-S已用于：
- 在G蛋白激活检测中评估蛋白酶激活受体4(PAR4)的功能
- 用于G蛋白α亚基(Gα)的荧光法鸟嘌呤核苷酸交换测定
- 监测磷脂酶Cγ2突变体的Rac家族小鸟苷三磷酸酶（GTP酶）2 (Rac2)刺激活性
Biochem/physiol Actions
鸟苷5′-O-(3-硫代三磷酸)(GTP-γ-S)可激活鸟嘌呤-核苷酸结合蛋白，可通过酶法缓慢水解。它对鸟苷三磷酸酶（GTP酶）的抑制作用强于鸟苷三磷酸(GTP)。
General description
鸟苷5′-O-(3-硫代三磷酸)四锂盐(GTP-γ-S)是GTP的不可水解类似物。它是光感受器中磷酸二酯酶的抑制剂。
Other Notes
仅用于生命科学研究。不可用于诊断。
Preparation Note
-15至-25°C环境下干燥储存粉末；pH值约为7。应在-15至-25°C下可稳定保存六个月。
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
Claudia Walliser et al.
The Journal of biological chemistry, 291(42), 22136-22148 (2016-08-21)
Mutations in the gene encoding phospholipase C-γ2 (PLCγ2) have been shown to be associated with resistance to targeted therapy of chronic lymphocytic leukemia (CLL) with the Bruton's tyrosine kinase inhibitor ibrutinib. The fact that two of these mutations, R665W and
S Nawy et al.
Neuron, 7(4), 677-683 (1991-10-01)
Transmitter release from photoreceptors is decreased by light, resulting in a conductance increase in depolarizing bipolar cells. Addition of exogenous cGMP through a patch pipette to depolarizing bipolar cells from slices of dark-adapted tiger salamander retina resulted in an enhancement
Stephen R Sprang
Biopolymers, 105(8), 449-462 (2016-03-22)
This review addresses the regulatory consequences of the binding of GTP to the alpha subunits (Gα) of heterotrimeric G proteins, the reaction mechanism of GTP hydrolysis catalyzed by Gα and the means by which GTPase activating proteins (GAPs) stimulate the
K Sasaki et al.
Nature, 325(6101), 259-262 (1987-01-15)
Recently, a GTP-binding protein sensitive to islet activating protein (IAP) has been suggested to be important in producing K+-currents when the muscarinic receptor of the atrial muscle is activated by acetylcholine (ACh). Here we confirm the blocking effects of IAP
Gilbert Di Paolo, Markus R. Wenk
Lipids, 3-18 (2012)
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