生物来源
bovine liver
质量水平
形式
lyophilized
比活
10 U/mg
~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.)
包装
pkg of 3,000 U
制造商/商品名称
Roche
浓度
≥10-20 % (w/w)
技术
activity assay: suitable
颜色
white
最佳pH
8
溶解性
water: 20 mg/mL, colorless
适用性
suitable for UV spectrophotometry and general use
UniProt登记号
应用
life science and biopharma
异质活性
ADH <0.00500%
LDH <0.00500%
MDH <0.00500%
运输
wet ice
储存温度
2-8°C
基因信息
bovine ... GLUD1(281785)
一般描述
GDH（谷氨酸脱氢酶）是含有449个残基的六聚体，其位于线粒体中。它可充当氨基酸氧化和尿素生成之间的分支酶。L-谷氨酸：NAD（P）+氧化还原酶参与脱氨。
Approximately 10 U/mg lyophilizate (120 U/mg enzyme protein) at +25°C with 2-oxoglutarate as the substrate, and ADP as the activator.
应用
在葡萄酒酵母发酵过程中，谷氨酸脱氢酶用于通过酶分析测定残留铵。它作为NAD（P）和NAD（P）H 辅助因子循环系统的组分。
质量
污染物：分别含有<0.005％的ADH、LDH和MDH
序列
GIDH（310,000至350,000）的最小活性结构是一个55,400D的六聚体亚基。该六聚体能够可逆地自组装形成具有多达八个六聚体(2,200 kD)的聚合物。
其他说明
仅用于生命科学研究。不可用于诊断。
WGK
WGK 1
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
动植物源性产品
Disruption of the cell wall integrity gene ECM33 results in improved fermentation by wine yeast
Jin Zhang
Metabolic engineering (2018)
T J Stillman et al.
Journal of molecular biology, 234(4), 1131-1139 (1993-12-20)
We have solved the structure of the binary complex of the glutamate dehydrogenase from Clostridium symbiosum with glutamate to 1.9 A resolution. In this complex, the glutamate side-chain lies in a pocket on the enzyme surface and a key determinant
P J O'Brien et al.
Laboratory animals, 36(3), 313-321 (2002-07-30)
In a recent study in rats, alanine aminotransferase (ALT), the preferred plasma biomarker of hepatocellular injury in rats, was ineffective at detecting marked hepatic necrosis produced by acetaminophen (Human and Experimental Toxicology 19, 277-83, 2000). In contrast, glutamate dehydrogenase (GLDH)
G Hughes et al.
Analytica chimica acta, 933, 82-88 (2016-08-09)
A reagentless glutamate biosensor was applied to the determination of glutamate released from liver hepatocellular carcinoma cells (HepG2) in response to toxic challenge from various concentrations of paracetamol. A screen printed carbon electrode (SPCE) containing the electrocatalyst Meldola's Blue (MB-SPCE)
Po Chen et al.
Fly, 14(1-4), 3-9 (2020-07-04)
Glutamine: fructose-6-phosphate amidotransferase (GFAT) enzymes catalyse the first committed step of the hexosamine biosynthesis pathway (HBP) using glutamine and fructose-6-phosphate to form glucosamine-6-phosphate (GlcN6P). Numerous species (e.g. mouse, rat, zebrafish, chicken) including humans and Drosophila encode two broadly expressed copies
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