产品名称
谷氨酸脱氢酶(GlDH), from beef liver
biological source
bovine liver
form
lyophilized
specific activity
10 U/mg
~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.)
packaging
pkg of 3,000 U
manufacturer/tradename
Roche
concentration
≥10-20 % (w/w)
technique(s)
activity assay: suitable
color
white
optimum pH
8
solubility
water: 20 mg/mL, colorless
suitability
suitable for UV spectrophotometry and general use
UniProt accession no.
application(s)
life science and biopharma
foreign activity
ADH <0.00500%
LDH <0.00500%
MDH <0.00500%
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Gene Information
bovine ... GLUD1(281785)
Analysis Note
污染物：分别含有<0.005％的ADH、LDH和MDH
Application
在葡萄酒酵母发酵过程中，谷氨酸脱氢酶用于通过酶分析测定残留铵。它作为NAD（P）和NAD（P）H 辅助因子循环系统的组分。
General description
GDH（谷氨酸脱氢酶）是含有449个残基的六聚体，其位于线粒体中。它可充当氨基酸氧化和尿素生成之间的分支酶。L-谷氨酸：NAD（P）+氧化还原酶参与脱氨。
在+25°C下，以2-氧代戊二酸为底物、ADP为激活剂时，冻干物酶活性约为10 U/mg（120 U/mg酶蛋白）。
Other Notes
GIDH（310,000至350,000）的最小活性结构是一个55,400D的六聚体亚基。该六聚体能够可逆地自组装形成具有多达八个六聚体(2,200 kD)的聚合物。
仅用于生命科学研究。不可用于诊断。
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
低风险生物材料
动植物源性产品
此项目有
G Hughes et al.
Analytica chimica acta, 933, 82-88 (2016-08-09)
A reagentless glutamate biosensor was applied to the determination of glutamate released from liver hepatocellular carcinoma cells (HepG2) in response to toxic challenge from various concentrations of paracetamol. A screen printed carbon electrode (SPCE) containing the electrocatalyst Meldola's Blue (MB-SPCE)
T J Stillman et al.
Journal of molecular biology, 234(4), 1131-1139 (1993-12-20)
We have solved the structure of the binary complex of the glutamate dehydrogenase from Clostridium symbiosum with glutamate to 1.9 A resolution. In this complex, the glutamate side-chain lies in a pocket on the enzyme surface and a key determinant
Disruption of the cell wall integrity gene ECM33 results in improved fermentation by wine yeast
Jin Zhang
Metabolic engineering (2018)
P J O'Brien et al.
Laboratory animals, 36(3), 313-321 (2002-07-30)
In a recent study in rats, alanine aminotransferase (ALT), the preferred plasma biomarker of hepatocellular injury in rats, was ineffective at detecting marked hepatic necrosis produced by acetaminophen (Human and Experimental Toxicology 19, 277-83, 2000). In contrast, glutamate dehydrogenase (GLDH)
Po Chen et al.
Fly, 14(1-4), 3-9 (2020-07-04)
Glutamine: fructose-6-phosphate amidotransferase (GFAT) enzymes catalyse the first committed step of the hexosamine biosynthesis pathway (HBP) using glutamine and fructose-6-phosphate to form glucosamine-6-phosphate (GlcN6P). Numerous species (e.g. mouse, rat, zebrafish, chicken) including humans and Drosophila encode two broadly expressed copies
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