生物来源
bacteriophage MS2
质量水平
形式
solution
分子量
~1200 kDa
包装
pkg of 500 μL (10 A260 units)
制造商/商品名称
Roche
技术
RNA extraction: suitable
颜色
colorless
溶解性
water: miscible
储存温度
−20°C (−15°C to −25°C)
一般描述
MS2噬菌体RNA由3569个核苷酸组成。MS2噬菌体在分子生物学研究中是模式系统，可用于病毒RNA复制，感染细胞翻译和生理学研究。MS2 RNA编码三个病毒多肽链，即蛋白质A、外壳蛋白和RNA复制酶复合物。蛋白质A和外壳蛋白组成了MS2病毒的结构。
应用
RNA，MS2用于：
- 评估RNA定量方法的标准品
- RNA提取
- 单管荧光PERT检测（STF-PERT），对不同逆转录病毒类型进行准确定量
- 各种研究，在体内或体外使用天然RNA的蛋白合成系统，起始、延长或终止多肽链合成。
- 结构和功能研究。
- 在cDNA合成过程中稳定RNA
- 在RNA提取或分离过程中提高RNA产量
质量
典型分析：根据现在的质量控制步骤，不会有蛋白质和宿主核酸。
序列
链长度3569个核苷酸
制备说明
工作溶液：Storage buffer: 10 mM Tris-HCl，1 mM EDTA，pH 7.0。
其他说明
仅用于生命科学研究。不可用于诊断。
储存分类代码
12 - Non Combustible Liquids
WGK
nwg
闪点(°F)
No data available
闪点(°C)
No data available
法规信息
常规特殊物品
Monique I Andersson et al.
Wellcome open research, 5, 181-181 (2020-12-09)
Background: Laboratory diagnosis of SARS-CoV-2 infection (the cause of COVID-19) uses PCR to detect viral RNA (vRNA) in respiratory samples. SARS-CoV-2 RNA has also been detected in other sample types, but there is limited understanding of the clinical or laboratory
Kirstine Kirkegaard et al.
Journal of assisted reproduction and genetics, 37(9), 2199-2209 (2020-07-19)
miRNAs have been suggested as biomarkers of embryo viability; however, findings from preliminary studies are divergent. Furthermore, the presence of other types of small RNA molecules remains to be investigated. The purpose of this study was to perform a comprehensive
Dennis M Timmerman et al.
Cancers, 13(17) (2021-09-11)
MicroRNAs (miRNAs) are short, non-coding RNAs involved in translation regulation. Dysregulation has been identified in cancer cells. miRNAs can be secreted and detectable in body fluids; therefore, they are potential liquid-biopsy biomarkers. The miR-371a-3 cluster members are an example, monitoring
Michael Eikmans et al.
International journal of molecular sciences, 21(11) (2020-06-12)
About 10-15% of couples who want to conceive suffer from subfertility, while in 30% of these cases, a male factor plays a role. Levels of particular microRNAs in seminal plasma, including those involved in spermatogenesis, may serve as an indicative
Shalini Das Gupta et al.
Scientific reports, 10(1), 9012-9012 (2020-06-04)
Quantification of plasma microRNAs (miRNAs) as non-invasive disease biomarkers is subject to multiple technical variabilities. This study aimed to develop an optimized protocol for miRNA quantification from rodent plasma. We hypothesized that a fixed small RNA concentration input for reverse
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