产品名称
RNA, MS2, from bacteriophage MS2
biological source
bacteriophage MS2
form
solution
mol wt
~1200 kDa
packaging
pkg of 500 μL (10 A260 units)
manufacturer/tradename
Roche
technique(s)
RNA extraction: suitable
color
colorless
solubility
water: miscible
storage temp.
−20°C (−15°C to −25°C)
Quality Level
Analysis Note
典型分析：根据现在的质量控制步骤，不会有蛋白质和宿主核酸。
Application
RNA，MS2用于：
- 评估RNA定量方法的标准品
- RNA提取
- 单管荧光PERT检测（STF-PERT），对不同逆转录病毒类型进行准确定量
- 各种研究，在体内或体外使用天然RNA的蛋白合成系统，起始、延长或终止多肽链合成。
- 结构和功能研究。
- 在cDNA合成过程中稳定RNA
- 在RNA提取或分离过程中提高RNA产量
General description
MS2噬菌体RNA由3569个核苷酸组成。MS2噬菌体在分子生物学研究中是模式系统，可用于病毒RNA复制，感染细胞翻译和生理学研究。MS2 RNA编码三个病毒多肽链，即蛋白质A、外壳蛋白和RNA复制酶复合物。蛋白质A和外壳蛋白组成了MS2病毒的结构。
Other Notes
仅用于生命科学研究。不可用于诊断。
链长度3569个核苷酸
Preparation Note
工作溶液：Storage buffer: 10 mM Tris-HCl，1 mM EDTA，pH 7.0。
存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
No data available
flash_point_c
No data available
法规信息
常规特殊物品
此项目有
Roman Aranda et al.
Analytical biochemistry, 387(1), 122-127 (2009-05-21)
Quantification of RNA is essential for various molecular biology studies. In this work, three quantification methods were evaluated: ultraviolet (UV) absorbance, microcapillary electrophoresis (MCE), and fluorescence-based quantification. Viral, bacterial, and eukaryotic RNA were measured in the 500 to 0.05-ng microl(-1)
Niina Vuokila et al.
Neuroscience, 433, 21-35 (2020-03-07)
Mechanisms initiated by traumatic brain injury (TBI), leading to the development of progressive secondary injury are poorly understood. MicroRNAs (miRNAs) have a proposed role in orchestrating the post-injury aftermath as a single miRNA can control the expression of several genes.
Thomas C Roberts et al.
Biological procedures online, 16(1), 5-5 (2014-03-19)
MicroRNAs (miRNAs) are short RNA molecules which regulate gene expression in eukaryotic cells, and are abundant and stable in biofluids such as blood serum and plasma. As such, there has been heightened interest in the utility of extracellular miRNAs as
Rom S Leidner et al.
PloS one, 8(3), e57841-e57841 (2013-03-09)
Genome-wide platforms for high-throughput profiling of circulating miRNA (oligoarray or miR-Seq) offer enormous promise for agnostic discovery of circulating miRNA biomarkers as a pathway for development in breast cancer detection. By harmonizing data from 15 previous reports, we found widespread
Shalini Das Gupta et al.
Scientific reports, 10(1), 9012-9012 (2020-06-04)
Quantification of plasma microRNAs (miRNAs) as non-invasive disease biomarkers is subject to multiple technical variabilities. This study aimed to develop an optimized protocol for miRNA quantification from rodent plasma. We hypothesized that a fixed small RNA concentration input for reverse
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