生物来源
pig gastric mucosa
质量水平
形式
lyophilized (salt-free)
比活
~2500 units/mg protein
包装
pkg of 1 g
制造商/商品名称
Roche
最佳pH
1.8-2.2
运输
wet ice
储存温度
2-8°C
一般描述
胃蛋白酶是一种来自猪胃粘膜的天冬氨酸内蛋白酶。它主要用于对酸性介质中的蛋白质和多肽进行非特异性水解，还能促进天然免疫球蛋白的限制性酶解，产生生物活性片段。
特异性
天冬氨酸内肽酶具有相对广泛的特异性。优先裂解：P1和P1′ 位置的疏水性残基，其中芳香族优先。
应用
胃蛋白酶已用于消化大鼠肝脏组织。
制备说明
工作浓度：0.05至0.5 mg/ml
储存条件（工作溶液）：在中性pH和无菌条件下，胃蛋白酶溶液可以在2至8℃下稳定储存至少1周。冷冻的酶溶液分装品可能更稳定。
溶剂为10mM HCl（5-10mg/ml）的储备溶液也可以在2至8℃下稳定储存至少1周。避光、密闭保存。
在40℃下短暂（2分钟）孵育对冻干胃蛋白酶或胃蛋白酶溶液的活性的影响可忽略不计。
储存条件（工作溶液）：在中性pH和无菌条件下，胃蛋白酶溶液可以在2至8℃下稳定储存至少1周。冷冻的酶溶液分装品可能更稳定。
溶剂为10mM HCl（5-10mg/ml）的储备溶液也可以在2至8℃下稳定储存至少1周。避光、密闭保存。
在40℃下短暂（2分钟）孵育对冻干胃蛋白酶或胃蛋白酶溶液的活性的影响可忽略不计。
分析说明
At 37 °C with hemoglobin as the substrate. One unit is the enzyme activity which liberates the amount of Tyr producing an increase in the absorbance of 0.001/minute at 280 nm.
其他说明
仅用于生命科学研究。不可用于诊断。
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
动植物源性产品
Lauren M Ashwood et al.
BMC biology, 21(1), 121-121 (2023-05-25)
The ShK toxin from Stichodactyla helianthus has established the therapeutic potential of sea anemone venom peptides, but many lineage-specific toxin families in Actiniarians remain uncharacterised. One such peptide family, sea anemone 8 (SA8), is present in all five sea anemone superfamilies.
Ganesan Arunkumar et al.
Science advances, 8(9), eabl5621-eabl5621 (2022-03-03)
Chromosome instability is a critical event in cancer progression. Histone H3 variant CENP-A plays a fundamental role in defining centromere identity, structure, and function but is innately overexpressed in several types of solid cancers. In the cancer background, excess CENP-A
Marie van Dijk et al.
Prenatal diagnosis, 42(13), 1612-1621 (2022-11-08)
Noninvasive Prenatal Diagnosis has recently been introduced for a limited number of monogenetic disorders. However, the majority of DNA diagnostics still require fetal material obtained using an invasive test. Recently, a novel technique, TRIC (Trophoblast Retrieval and Isolation from the
William D Kim et al.
Frontiers in genetics, 13, 1045738-1045738 (2022-11-29)
Mutations in CLN5 cause a subtype of neuronal ceroid lipofuscinosis (NCL) called CLN5 disease. The NCLs, commonly referred to as Batten disease, are a family of neurodegenerative lysosomal storage diseases that affect all ages and ethnicities globally. Previous research showed
Claus Kordes et al.
The Journal of clinical investigation, 124(12), 5503-5515 (2014-11-18)
Retinoid-storing hepatic stellate cells (HSCs) have recently been described as a liver-resident mesenchymal stem cell (MSC) population; however, it is not clear whether these cells contribute to liver regeneration or serve as a progenitor cell population with hepatobiliary characteristics. Here
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门