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生物来源
Staphylococcus aureus
质量水平
表单
lyophilized
比活
~15,000 U/mg
包装
pkg of 15,000 U
制造商/商品名称
Roche
最佳pH
7.5
运输
wet ice
应用
- 从体外翻译系统中去除内源性RNA
- RNA测序实验
核酸酶S7已用于核糖体分析的核糖核酸酶选择过程研究。
生化/生理作用
核酸酶S7是一种核酸内切酶，主要切割单链底物。它还切割双链DNA或RNA。它切割RNA和DNA的5′磷酸二酯键，产生3′-单核苷酸和寡核苷酸。酶的活性取决于Ca2+ ，添加EDTA可使酶完全灭活。相比于DNA上的GC，核酸酶S7切割对富含AT的区域高度特异。
单位定义
一单位酶释放的足够量的酸溶性寡核苷酸可使A260增加1.0。
储存及稳定性
未开封试剂储存于2-8 °C；1 mg/mL的稀释液则储存于-15 to -25 °C。
其他说明
仅用于生命科学研究。不可用于诊断。
警示用语：
Danger
危险分类
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
靶器官
Respiratory system
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
does not flash
闪点(°C)
does not flash
法规信息
常规特殊物品
Ariane Lismer et al.
Nucleic acids research, 48(20), 11380-11393 (2020-10-18)
Advancing the molecular knowledge surrounding fertility and inheritance has become critical given the halving of sperm counts in the last 40 years, and the rise in complex disease which cannot be explained by genetics alone. The connection between both these
Maxim V Gerashchenko et al.
Nucleic acids research, 49(2), e9-e9 (2020-12-03)
There has been a surge of interest towards targeting protein synthesis to treat diseases and extend lifespan. Despite the progress, few options are available to assess translation in live animals, as their complexity limits the repertoire of experimental tools to
Ribonuclease selection for ribosome profiling.
Gerashchenko M V and Gladyshev V N
Nucleic Acids Research, 45(2), e6-e6 (2016)
Reimo Zoschke et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(13), E1678-E1687 (2015-03-17)
Chloroplast genomes encode ∼ 37 proteins that integrate into the thylakoid membrane. The mechanisms that target these proteins to the membrane are largely unexplored. We used ribosome profiling to provide a comprehensive, high-resolution map of ribosome positions on chloroplast mRNAs
High sequence specificity of micrococcal nuclease.
Dingwall C, et al.
Nucleic Acids Research, 9(12), 2659-2674 (1981)
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