biological source
Staphylococcus aureus
form
lyophilized
specific activity
~15,000 U/mg
packaging
pkg of 15,000 U
manufacturer/tradename
Roche
optimum pH
7.5
shipped in
wet ice
Application
- 从体外翻译系统中去除内源性RNA
- RNA测序实验
核酸酶S7已用于核糖体分析的核糖核酸酶选择过程研究。
Biochem/physiol Actions
核酸酶S7是一种核酸内切酶，主要切割单链底物。它还切割双链DNA或RNA。它切割RNA和DNA的5′磷酸二酯键，产生3′-单核苷酸和寡核苷酸。酶的活性取决于Ca2+ ，添加EDTA可使酶完全灭活。相比于DNA上的GC，核酸酶S7切割对富含AT的区域高度特异。
Preparation Note
未开封试剂储存于2-8 °C；1 mg/mL的稀释液则储存于-15 to -25 °C。
Other Notes
一单位酶释放的足够量的酸溶性寡核苷酸可使A260增加1.0。
仅用于生命科学研究。不可用于诊断。
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - Skin Sens. 1 - STOT SE 3
target_organs
Respiratory system
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
does not flash
flash_point_c
does not flash
法规信息
常规特殊物品
此项目有
Maxim V Gerashchenko et al.
Nucleic acids research, 49(2), e9-e9 (2020-12-03)
There has been a surge of interest towards targeting protein synthesis to treat diseases and extend lifespan. Despite the progress, few options are available to assess translation in live animals, as their complexity limits the repertoire of experimental tools to
Ribonuclease selection for ribosome profiling.
Gerashchenko M V and Gladyshev V N
Nucleic Acids Research, 45(2), e6-e6 (2016)
Ariane Lismer et al.
Nucleic acids research, 48(20), 11380-11393 (2020-10-18)
Advancing the molecular knowledge surrounding fertility and inheritance has become critical given the halving of sperm counts in the last 40 years, and the rise in complex disease which cannot be explained by genetics alone. The connection between both these
Reimo Zoschke et al.
Proceedings of the National Academy of Sciences of the United States of America, 112(13), E1678-E1687 (2015-03-17)
Chloroplast genomes encode ∼ 37 proteins that integrate into the thylakoid membrane. The mechanisms that target these proteins to the membrane are largely unexplored. We used ribosome profiling to provide a comprehensive, high-resolution map of ribosome positions on chloroplast mRNAs
Organ-specific translation elongation rates measured by in vivo ribosome profiling.
Gerashchenko M V, et al.
bioRxiv, 279257 (2018)
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