产品名称
Ultrafree®离心过滤器，0.5 mL样品体积, pore size 0.65 μm, PVDF membrane (hydrophilic), sterile
material
PVDF membrane (hydrophilic)
polypropylene
sterility
sterile
feature
holdup volume 5 μL
packaging
pkg of 50 ea
manufacturer/tradename
Ultrafree®
parameter
0.5 mL sample volume
50 °C max. temp.
technique(s)
protein extraction: suitable
protein purification: suitable
L
45 mm
diam.
10.6 mm
filtration area
0.2 cm2
working volume
0.5 mL
matrix
Durapore®
pore size
0.65 μm pore size
Quality Level
application(s)
sample preparation
shipped in
ambient
Application
Ultrafree®-MC离心过滤器用于：
- 过滤精浆中的细胞外囊泡(EV)
- 纯化单链选择性尿嘧啶-DNA糖基酶(SMUG1)
- 纯化EV以进行纳米颗粒跟踪分析
General description
Ultrafree®-MC离心过滤器是一种一次性过滤器，用于去除生物水溶液中的颗粒物。
滤器类型：微滤
滤器编码：DVPP
Physical form
色码：紫色
Legal Information
Durapore is a registered trademark of Merck KGaA, Darmstadt, Germany
Ultrafree is a registered trademark of Merck KGaA, Darmstadt, Germany
Ruth E Thomas et al.
PLoS genetics, 14(9), e1007694-e1007694 (2018-09-27)
Mutations in the glucosylceramidase beta (GBA) gene are strongly associated with neurodegenerative diseases marked by protein aggregation. GBA encodes the lysosomal enzyme glucocerebrosidase, which breaks down glucosylceramide. A common explanation for the link between GBA mutations and protein aggregation is
Janis A Müller et al.
Nature communications, 9(1), 2207-2207 (2018-06-09)
Zika virus (ZIKV) causes severe birth defects and can be transmitted via sexual intercourse. Semen from ZIKV-infected individuals contains high viral loads and may therefore serve as an important vector for virus transmission. Here we analyze the effect of semen
Penelope Kroustallaki et al.
Cell reports, 28(7), 1690-1702 (2019-08-15)
Telomerase biogenesis is a complex process where several steps remain poorly understood. Single-strand-selective uracil-DNA glycosylase (SMUG1) associates with the DKC1-containing H/ACA ribonucleoprotein complex, which is essential for telomerase biogenesis. Herein, we show that SMUG1 interacts with the telomeric RNA component
实验方案
Affinity interaction chromatography is an effective method for protein purification that can achieve up to 95% purity in one step
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