产品名称
Anti-TOMM20 Mouse mAb (4F3), liquid, clone 4F3, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
4F3, monoclonal
form
liquid
does not contain
preservative
species reactivity
human, rat, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
dilution
(ELISA (see coments)
Immunoblotting (1-5 µg/mL)
Immunocytochemistry (10 µg/mL)
Paraffin Sections (3 µg/mL))
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TOMM20(9804)
Analysis Note
Negative Control
293T cells
293T cells
Positive Control
HeLa cells, PC12 cells, NIH3T3 cells, Human small intestine tissue
HeLa cells, PC12 cells, NIH3T3 cells, Human small intestine tissue
Application
ELISA (see coments)
Immunoblotting (1-5 g/ml)
Immunocytochemistry (10 g/ml)
Paraffin Sections (3 g/ml)
Disclaimer
Toxicity: Regulatory Review (Z)
General description
Purified mouse monoclonal antibody. Recognizes the ~12-16 kDa TOMM20 protein.
Recognizes the ~12-16 kDa TOMM20 protein in HeLa, PC12, and NIH3T3 cells and human small intestine tissue.
This Anti-TOMM20 Mouse mAb (4F3) is validated for use in ELISA, Immunoblotting, Immunocytochemistry, Paraffin Sections for the detection of TOMM20.
Immunogen
Full-length, recombinant, human TOMM20 (aa 1-146), expressed as a GST fusion protein
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Adam Z Spitz et al.
Nature communications, 12(1), 1134-1134 (2021-02-20)
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Nature neuroscience, 26(5), 737-750 (2023-04-25)
Pathological hallmarks of Alzheimer's disease (AD) precede clinical symptoms by years, indicating a period of cognitive resilience before the onset of dementia. Here, we report that activation of cyclic GMP-AMP synthase (cGAS) diminishes cognitive resilience by decreasing the neuronal transcriptional
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