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一般描述
Anti-AFP, mouse monoclonal, clone 1G7, recognizes ~70-75 kDa AFP protein in HepG2 cells. It is validated for ELISA, Western blotting, and immunocytochemistry.
Purified mouse monoclonal antibody. Recognizes the ~70-75 kDa AFP protein.
Recognizes the ~70-75 kDa AFP protein in HepG2 cells.
免疫原
A recombinant polypeptide corresponding to amino acids of 500-610 human AFP, expressed as a GST fusion protein
应用
ELISA (see coments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 µg/ml)
警告
Toxicity: Regulatory Review (Z)
外形
In PBS, pH 7.4.
重悬
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Negative Control
293T cells
293T cells
Positive Control
HepG2 cells
HepG2 cells
其他说明
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
12 - Non Combustible Liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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