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Merck
CN
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主要文件

安全信息

ST1629

Sigma-Aldrich

Anti-GNA13 Mouse mAb (6F6-B5)

liquid, clone 6F6-B5, Calbiochem®

别名:

Anti-G Protein α13, Anti-Guanine Nucleotide Binding Protein α13, Anti-Guanine Nucleotide Binding Protein α13, Anti-G Protein α13

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About This Item

UNSPSC代码:
12352203
NACRES:
NA.41

生物来源

mouse

质量水平

100

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

6F6-B5, monoclonal

表单

liquid

不包含

preservative

种属反应性

human

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

同位素/亚型

IgG1

运输

wet ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... GNA13(10672)

一般描述

Anti-GNA13, mouse monoclonal, clone 6F6-B5, recognizes the ~40-45 kDa GNA13 protein in HepG2 cells. It is validated for use in ELISA and Western blotting.
Purified mouse monoclonal antibody. Recognizes the ~40-45 kDa GNA13 protein.
Recognizes the ~40-45 kDa GNA13 protein in HepG2 cells.

免疫原

Full-length, recombinant, human GNA13 (aa 1-378), expressed as a GST fusion protein

应用


ELISA (see comments)
Immunoblotting (1-5 g/ml)

警告

Toxicity: Regulatory Review (Z)

外形

In PBS, pH 7.2.

重悬

Following initial thaw, aliquot and freeze (-20°C).

分析说明

Negative Control
293T cells
Positive Control
HepG2 cells

其他说明

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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储存分类代码

12 - Non Combustible Liquids

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

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Suhail Ahmed Kabeer Rasheed et al.
The Journal of biological chemistry, 288(11), 7986-7995 (2013-01-19)
G protein-coupled receptors (GPCRs) and their ligands have been implicated in progression and metastasis of several cancers. GPCRs signal through heterotrimeric G proteins, and among the different types of G proteins, GNA12/13 have been most closely linked to tumor progression.
Suhail Ahmed Kabeer Rasheed et al.
Oncogene, 37(10), 1340-1353 (2017-12-20)
Treatment failure in solid tumors occurs due to the survival of specific subpopulations of cells that possess tumor-initiating (TIC) phenotypes. Studies have implicated G protein-coupled-receptors (GPCRs) in cancer progression and the acquisition of TIC phenotypes. Many of the implicated GPCRs
Suhail Ahmed Kabeer Rasheed et al.
Molecular cancer, 14, 67-67 (2015-04-19)
Gα13 (GNA13) is the α subunit of a heterotrimeric G protein that mediates signaling through specific G protein-coupled receptors (GPCRs). Our recent study showed that control of GNA13 expression by specific microRNAs (miRNAs or miRs) is important for prostate cancer

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