一般描述
Purified mouse monoclonal antibody. Recognizes the ~67 kDa VCP protein.
Recognizes the ~67 kDa VCP protein in HePG2 cells and human placenta tissue.
This Anti-VCP Mouse mAb (4A8) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of VCP.
免疫原
Full-length, recombinant human VCP (aa 1-589), expressed as a GST fusion protein
应用
ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (3 g/ml)
警告
Toxicity: Regulatory Review (Z)
外形
In PBS, pH 7.2.
重悬
Following initial thaw, aliquot and freeze (-20°C).
分析说明
Positive Control
HePG2 cells, human placenta tissue
HePG2 cells, human placenta tissue
其他说明
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
12 - Non Combustible Liquids
WGK
nwg
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
新产品
Philipp Voisard et al.
International journal of molecular sciences, 23(12) (2022-06-25)
Valosin-containing protein (VCP) acts as a key regulator of cellular protein homeostasis by coordinating protein turnover and quality control. Mutations in VCP lead to (cardio-)myopathy and neurodegenerative diseases such as inclusion body myopathy with Paget's disease of the bone and
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