生物来源
mouse
质量水平
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
4C1.3, monoclonal
形式
liquid
不包含
preservative
种属反应性
mouse, human, rat
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG
储存温度
−70°C
靶向翻译后修饰
unmodified
基因信息
human ... PPARGC1A(10891)
一般描述
PGC-1α是PPARα、PPARγ和其他转录因子的共激活因子，可调节棕色脂肪组织中适应性生热、肝/肾糖异生和肌肉纤维类型转换的转录程序。全长蛋白质为113 kDa，通过冷暴露在棕色脂肪组织中诱导，通过禁食在肝脏和肾脏中诱导，并通过运动在骨骼肌中诱导。全长基因的可变剪接产生270aa的N末端剪接变体，其在〜38kDa处迁移并由诱导全长蛋白质表达的相同生理信号诱导。N末端PGC-1α剪接变体也受到翻译后修饰的影响，从而改变其迁移和表观分子量。
蛋白G纯化的小鼠单克隆抗体。识别内源形式的PGC-1α，包括~113 kDa PGC-1α蛋白和~38 kDa剪接变体。
识别棕色脂肪组织、肝脏和肾脏中的内源形式的PGC-1α，包括~113 kDa PGC-1α蛋白和~38 kDa剪接变体。
该抗PGC-1α小鼠单克隆抗体（4C1.3）经验证可用于免疫印迹、免疫细胞化学、免疫沉淀、石蜡切片以检测PGC-1α。
免疫原
小鼠
由小鼠PGC-1的1-120位氨基酸组成的重组蛋白α
应用
免疫印迹（1 µg/mL）
免疫细胞化学（5-10 µg/mL）
免疫沉淀（10 µg/mL）
石蜡切片（整个安装）（请参阅应用参考文献）
免疫细胞化学（5-10 µg/mL）
免疫沉淀（10 µg/mL）
石蜡切片（整个安装）（请参阅应用参考文献）
警告
毒性：多个毒性值，请参阅MSDS（O）
外形
1小瓶100 µg抗体溶于50 mM PBS中，参见批次特定浓度的小瓶。一小瓶NT-PGC-1α阳性对照，在含有10 mM &-巯基乙醇和1%SDS的RIPA缓冲液中以25 µl全细胞提取物的形式提供；每个泳道上样10 µl；在SDS-PAGE上样前加入样品缓冲液。
重悬
初次解冻后，分装冻存（-70°C）。
其他说明
Lai, L., et al. 2008.Genes Dev.14, 1948.
Rodgers, J.T., et al. 2008.FEBS Lett.582, 46.
Mazzucotelli, A., et al. 2007.Diabetes 10, 2467.
Nemoto, S., et al. 2005.J. Biol. Chem. 16, 16456.
Rodgers, J.T., et al. 2008.FEBS Lett.582, 46.
Mazzucotelli, A., et al. 2007.Diabetes 10, 2467.
Nemoto, S., et al. 2005.J. Biol. Chem. 16, 16456.
内源性PGC-1α主要存在于细胞核中，NT-PGC-1α存在于胞质和核部分中。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Not finding the right product?
Try our 产品选型工具.
WGK
WGK 2
Amy E Mendham et al.
Journal of applied physiology (Bethesda, Md. : 1985), 121(6), 1326-1334 (2016-10-16)
This study assessed the mitochondrial related signaling responses to a single bout of noncontact, modified football (touch rugby), played as small-sided games (SSG), or cycling (CYC) exercise in sedentary, obese, middle-aged men. In a randomized, crossover design, nine middle-aged, sedentary
Naoki Horii et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 312(4), R520-R528 (2017-01-27)
The purpose of this study was to investigate the effect of chronic chlorella intake alone or in combination with high-intensity intermittent exercise (HIIE) training on exercise performance and muscle glycolytic and oxidative metabolism in rats. Forty male Sprague-Dawley rats were
Filippe Falcão-Tebas et al.
The Journal of physiology, 597(1), 121-136 (2018-11-09)
A paternal high-fat diet/obesity before mating can negatively influence the metabolism of offspring. Exercise only early in life has a remarkable effect with respect to reprogramming adult rat offspring exposed to detrimental insults before conception. Exercise only early in life
Sudipta Chakrabarti et al.
Neurobiology of disease, 127, 362-373 (2019-04-01)
The late-infantile Batten disease or late-infantile neuronal ceroid lipofuscinosis (LINCL) is an autosomal recessive lysosomal storage disorder caused by mutations in the Cln2 gene leading to deficiency of lysosomal enzyme tripeptidyl peptidase 1 (TPP1). At present, available options for this
Tess V Dupre et al.
Biochemical pharmacology, 169, 113644-113644 (2019-09-23)
A hallmark of acute kidney injury (AKI) is vascular rarefication and mitochondrial dysfunction. Promoting vascular recovery following AKI could facilitate kidney repair as the vasculature is responsible for oxygen and nutrient delivery to extravascular tissues. Little is known about mitochondrial
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门