推荐产品
生物来源
mouse
质量水平
抗体形式
unpurified
抗体产品类型
primary antibodies
克隆
FK2, monoclonal
形式
liquid
包含
≤0.1% sodium azide as preservative
种属反应性（根据同源性预测）
all
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG1
运输
wet ice
储存温度
−20°C
靶向翻译后修饰
unmodified
一般描述
可识别K29-、K48-和K63-连接的多泛素化和单泛素化蛋白，但不能识别Raji细胞中的游离泛素。
抗泛素小鼠mAb（FK2）经过验证可用于ELISA、免疫印迹、免疫沉淀和免疫荧光法，以检测泛素。
通过使用指定的免疫原免疫BALB/c小鼠并将脾细胞与骨髓瘤细胞融合而产生的小鼠单克隆抗体。可识别K29-、K48-和K63-连接的多泛素化和单泛素化蛋白。
免疫原
人
未纯化的多聚泛素化溶菌酶
应用
ELISA（参见应用参考文献）
免疫印迹法（1:1000）
免疫沉淀法（参见应用参考文献和备注）
免疫荧光法（见应用参考文献）
包装
请参考特定浓度批号的标签。
警告
毒性：标准处理（A）
外形
溶于PBS中。
制备说明
Raji细胞
重悬
初次融化后，等分并冷冻（-20°C）。稀释抗体可在4°C下储存最长1个月。
其他说明
Matsumoto, M., et al. 2005.Proteomics 5, 4545.
Boname, J.N., et al. 2001.Immunity 15, 627.
Campbell, V., et al. 2001.Neuron 32, 1013.
Fujimuro, M., et al. 2003.FEBS Letts.349, 173.
Boname, J.N., et al. 2001.Immunity 15, 627.
Campbell, V., et al. 2001.Neuron 32, 1013.
Fujimuro, M., et al. 2003.FEBS Letts.349, 173.
不能识别游离泛素。对于免疫沉淀，一旦抗体被固定，它就显示出对游离泛素以及多泛素化和单泛素化蛋白的亲和力。在单个系统中，应对抗体进行滴定以获得最佳结果。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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