推荐产品
生物来源
rabbit
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
human, mouse
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
同位素/亚型
IgG
储存温度
2-8°C
靶向翻译后修饰
unmodified
基因信息
human ... TIAM1(7074)
一般描述
Immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~200 kDa Tiam1 protein.
Recognizes the ~200 kDa Tiam1 protein in Jurkat and NIH3T3 cells.
This Anti-Tiam1 Rabbit pAb is validated for use in Immunoblotting for the detection of Tiam1.
Tiam1 (T-cell lymphoma invasion and metastasis 1) was originally identified by its ability to induce invasion by T-lymphoma cells when amplified. Tiam1 is a specific guanine nucleotide exchange factor (GEF) for the GTPase, Rac. By activating Rac1, Tiam1 regulates many cellular functions including actin cytoskeleton dynamics, cell proliferation, and cell survival. Tiam1 activity is regulated by its translocation to the membrane and the subsequent binding of its PH domain to phosphatidylinositol-3,4,5-triphosphate. Tiam1 is cleaved in a caspase-dependent manner in apoptotic cells, rendering it inactive as a result of an inability to translocate to the plasma membrane.
免疫原
Human
a synthetic peptide corresponding to amino acids from the C-terminal domain of human Tiam1
应用
Immunoblotting (1:1000)
包装
Please refer to vial label for lot-specific concentration.
警告
Toxicity: Highly Toxic (H)
外形
In Tris-Citrate/phosphate, pH 7.0-8.0.
重悬
Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.
分析说明
Positive Control
Jurkat or NIH3T3 cells
Jurkat or NIH3T3 cells
其他说明
Antibody should be titrated for optimal results in individual systems.
Mertens, A.E., et al. 2003. FEBS Lett.546, 11.
Qi, H., et al. 2001. Cell Growth Differ.12, 603.
Stam, J.C., et al. 1997. J. Biol. Chem.272, 28447.
Qi, H., et al. 2001. Cell Growth Differ.12, 603.
Stam, J.C., et al. 1997. J. Biol. Chem.272, 28447.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
WGK
WGK 1
法规信息
新产品
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RORγt is a master transcription factor of Th17 cells and considered as a promising drug target for the treatment of autoimmune diseases. Here, we show the guanine nucleotide exchange factor, Tiam1, and its cognate Rho-family G protein, Rac1, regulate interleukin
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Exposure to psychostimulants results in structural and synaptic plasticity in striatal medium spiny neurons (MSNs). These cellular adaptations arise from alterations in genes that are highly implicated in the rearrangement of the actin-cytoskeleton, such as T-lymphoma invasion and metastasis 1
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