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Merck
CN
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文件

ST1049

Sigma-Aldrich

Anti-20S Proteasome α1, 2, 3, 5, 6, & 7-Subunits Mouse mAb (MCP231)

liquid, clone MCP231, Calbiochem®

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UNSPSC代码:
12352203

生物来源

mouse

质量水平

100

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

MCP231, monoclonal

形式

liquid

包含

≤0.1% sodium azide as preservative

种属反应性

human, yeast, rat, rabbit, mouse

制造商/商品名称

Calbiochem®

储存条件

OK to freeze
avoid repeated freeze/thaw cycles

同位素/亚型

IgG1

运输

wet ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... PSMA1(5682)

一般描述

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with mouse myleloma cells. Recognizes the ~29-32 kDa α1, 2, 3, 5, 6 and 7 subunits of the 20S proteasome.
Recognizes α1, 2, 3, 5, 6 and 7 subunits of 20S proteasome in human placental proteasome preparations.
This Anti-20S Proteasome alpha-1, 2, 3, 5, 6, & 7 Subunits Antibody is validated for use in WB, IP, ELISA, IF, ICC and IHC for the detection of 20S Proteasome alpha-1, 2, 3, 5, 6, & 7 Subunits.

免疫原

Epitope: within the prosbox I motif common to α subunits (TVWSPQGRLHQVEYAMEA)
dinitrophenylated proteasomes

应用


Immunoblotting (1:1000, chemiluminescence)
Immunoprecipitation (not recommended)
ELISA (see comments)
Immunofluorescence (see application references)
Immunocytochemistry (see application references)
Immunohistochemistry (see application references)
Frozen Sections (see application references)

警告

Toxicity: Highly Toxic (H)

分析说明

Positive Control
Human placental tissue

其他说明

Immunoblotting using a human placental proteasome preparation yields two bands at ~29 kDa and ~32 kDa. In ELISA the antibody reacts with the peptide sequence TVWSPQGRLHQVEYAMEA encompassing the prosbox I motif common to α-type subunits. Antibody should be titrated for optimal results in individual systems.
Kopp, F., et al. 1997. Proc. Natl. Acad. Sci. USA94, 2939.

法律信息

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

WGK

nwg

闪点(°F)

Not applicable

闪点(°C)

Not applicable

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Lin Lyu et al.
Autophagy, 1-16 (2021-08-13)
Until recently, the ubiquitin-proteasome system (UPS) and macroautophagy/autophagy were considered to be two independent systems that target proteins for degradation by proteasomes or via lysosomes, respectively. Here, we report that TRIM44 (tripartite motif containing 44) is a novel link that
Shuangwu Sun et al.
Open biology, 7(5) (2017-05-26)
Ubiquitin-like domain-containing C-terminal domain phosphatase 1 (UBLCP1), an FCP/SCP phosphatase family member, was identified as the first proteasome phosphatase. UBLCP1 binds to proteasome subunit Rpn1 and dephosphorylates the proteasome in vitro However, it is still unclear which proteasome subunit(s) are

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