manufacturer/tradename
SNAP id®
technique(s)
western blot: suitable
compatibility
for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blØk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blØk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blØk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents
detection method
chemiluminescent, colorimetric, fluorometric
shipped in
ambient
storage temp.
room temp
Quality Level
General description
Application
Legal Information
Disclaimer
实验方案
The SNAP i.d. 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on Western blots.
相关内容
Two attributes of the Amicon® Pro device make it a convenient device for preparing pure, labeled antibody. First, the device enables highly efficient buffer exchange via diafiltration with simultaneous sample concentration in a single 15 minute spin. Second, the entire workflow can be performed within a single device, reducing the potential for sample loss. In this report, we describe the successful use of the Amicon® Pro device for small–scale biotinylation of a target antibody. The biotinylated antibody was used for detection of protein immunoprecipitated from cell lysate.
There’s so much room for experimental variability in traditional immunodetection workflows. For your peace of mind – and ours – we designed the SNAP i.d.® 2.0 system to streamline your Western blot and immunohistochemistry experiments. The concept is simple: a vacuum-driven flow of blocking, antibody, and washing solutions reduces slide and membrane handling. That means a lot less shaking, dipping, pouring and waiting. And now you can process multiple blots and slides in parallel, so it’s easy to apply consistent conditions across experiments.
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