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Merck
CN

SNAP2RL

SNAP id® 2.0 印迹滚筒

New Blot Roller specially designed for use with the SNAP i.d. 2.0 system.

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UNSPSC Code:
41116010
NACRES:
NB.22
eCl@ss:
42029053
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manufacturer/tradename

SNAP id®

technique(s)

western blot: suitable

compatibility

for use with Commercially available blocking reagents, for use with Luminata Western HRP Substrates, for use with Nitrocellulose, for use with PVDF (Immobilon membranes), for use with blØk<TMSYMBOL></TMSYMBOL>-CH Buffer (cat. no. WBAVDCH01), for use with blØk<TMSYMBOL></TMSYMBOL>-FL Buffer (cat. no. WBAVDFL01), for use with blØk<TMSYMBOL></TMSYMBOL>-PO Buffer (cat. no. WBAVDP001), for use with commercially available detection reagents

detection method

chemiluminescent, colorimetric, fluorometric

shipped in

ambient

storage temp.

room temp

Quality Level

Application

专为与SNAP i.d. 2.0系统配合使用而设计的新型印迹滚筒。

Legal Information

SNAP id is a registered trademark of Merck KGaA, Darmstadt, Germany

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实验方案

Western blotting involves protein separation by gel electrophoresis, protein transfer to a polyvinylidene difluoride (PVDF) or nitrocellulose membrane, and detection by various methods.

The SNAP i.d. 2.0 Protein Detection System is the second generation of the SNAP i.d. method for detecting immunoreactive proteins on Western blots.

Western blot protocol details protein transfer from gels to nitrocellulose, crucial for immunoblotting procedures in research.

带有用于不同印迹过程工作流程步骤的Western blot实验方法,描述了蛋白从SDS聚丙烯酰胺凝胶(SDS-PAGE)到硝酸纤维素膜(NC膜)的电泳转移。也称为免疫印迹实验方法。

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Two attributes of the Amicon® Pro device make it a convenient device for preparing pure, labeled antibody. First, the device enables highly efficient buffer exchange via diafiltration with simultaneous sample concentration in a single 15 minute spin. Second, the entire workflow can be performed within a single device, reducing the potential for sample loss. In this report, we describe the successful use of the Amicon® Pro device for small–scale biotinylation of a target antibody. The biotinylated antibody was used for detection of protein immunoprecipitated from cell lysate.

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