BioTracker^™ Mitophagy Live Cell Probe

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SCT264 live cell mitophagy detection probe accumulates and is immobilized on intact mitochondria, which fuse to the lysosome after mitophagy is induced, causing the probe to fluoresce brightly. Mitophagy has been associated with the accumulation of dysfunctional mitochondria in diseases such Alzheimer′s and Parkinson′s.

Mitochondria play an essential role in cells for the production of energy. Mitophagy, or the removal of damaged mitochondria via autophagy, has been connected to Alzheimer s and Parkinson s disease induced by the accumulation of depolarized mitochondria. Mitophagy serves as a dedicated elimination system that removes mitochondria whose dysfunction may be caused by oxidative stress and DNA damage. Mitochondria destined for autophagy are sequestered into the autophagosome, fused to the lysosome, and digested.

The Mitophagy probe accumulates in intact mitochondria, where it is immobilized by chemical bonds and exhibits a weak fluorescence under normal conditions. When mitophagy is induced, the damaged mitochondria fuse with the lysosome, increasing fluorescence


SPECTRAL PROPERTIES
λex: 530 nm
λem: 700 nm

A 100 uM stock solution of the mitophagy probe is created by dissolving 50 uL DMSO to one tube (5 ug) of mitophagy dye.
A typical working solution concentration is 1 nM.

This far red live cell probe for mitophagy detection fluoresces brightly when damaged mitochondria enter the acidic environment of the lysosome.

purple solid

Store at 2 - 8 °C, protected from light.

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany