HaloTag AcidiFluor Orange Ligand

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HaloTag AcidiFluor ORANGE is an acidic pH indicator. It consists of AcidiFluor ORANGE dye fused with HaloTag ligand, which forms a covalent bond with HaloTag. It shows quite weak fluorescence at neutral pH, but shows strong fluorescence at acidic pH. Since it is cell impermeable reagent, it can be used to label membrane-associated HaloTag fused proteins just by mixing and then monitoring pH changes. Relatively photostable fluorescence of AcidiFluor ORANGE enables time lapseimaging of pH change and tracking of endocytic/exocytic vesicles for long-time period.

Spectral Properties:
Absorbance (max): 520nm
Emission (max): 565nm

Research Category:
Live Cell Dye Signaling
Neuroscience.

HaloTag is a registered tradeMark of Promega Corporation.
AcidiFluor is a trademark of Goryo Chemical Inc.