BioTracker^™ EP-HMRG Green Protease Live Cell Probe

BioTracker^™ EP-HMRG is nearly nonfluorescent at pH 7 or higher. It fluoresces by the reaction with proteases that specifically digest the EP (glutamic acid, proline) peptide sequence. A green fluorescent product is membrane permeable and localizes to the lysosome.To use the probe, warm the vial to room temperature and add 30 μL of DMSO or PBS to one vial to make 1 mM solution.Dilute the stock solution with a neutral buffer or culture medium to 1-2 uM and use the diluted solution immediately.SPECTRAL PROPERTIES:Excitation: 500 nmEmission: 524 nmFor fluorescence microscopy, blue excitation filter cubes for FITC or GFP are suitable.12110 EP-HMRG Live Cell Probe w temperaturze -20°C, wysuszoną i chronioną przed światłem. Przed otwarciem fiolki należy ją krótko odwirować, aby zebrać jej zawartość na dnie. UWAGA: Nie zaleca się przechowywania odtworzonej sondy. Aby uzyskać najlepsze wyniki, należy wyrzucić niewykorzystany roztwór sondy i przygotować nowy do każdego użycia.

Live cell imaging probe that reacts with the proteases that digest the peptide sequence of EP (glutamic acid, proline) to fluoresce green by fluorescence microscopy or flow cytometry.

Early detection of esophageal squamous cell carcinoma (ESCC) is an important prognosticator, but is difficult to achieve by conventional endoscopy. Since fluorescence-based visualization is considered a promising approach, we aimed to develop an activata

Store BioTracker^™ EP-HMRG Live Cell Probe at -20°C, desiccated and protected from light. Centrifuge vial briefly to collect contents at bottom of vial before opening.NOTE: Storage of reconstituted probe is not recommended. For best results, discard unused probe solution and make fresh for each use.

BioTracker is a trademark of Merck KGaA, Darmstadt, Germany

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.