BioTracker fVF2 Voltage Sensitive Dye

Optical methods for measuring biochemical and biophysical events in living cells provide a powerful approach for monitoring cellular physiology in a non-invasive and high-throughput manner. Changes in the electrochemical potential across the plasma membrane of cells profoundly shapes cellular physiology. Excitable cells, such as neurons and cardiomyocytes, employ a consortium of ion channels both to maintain tight control over their membrane potential (Vm) and to initiate and propagate rapid changes in Vm. Rapid changes in Vm such as an action potential (AP) drive the unique physiology of these excitable cells. Disruption of the frequency, timing, and/or shape of APs is linked to serious human diseases ranging from epilepsy to long QT syndrome. The gold standard for measuring Vm and APs in live cells is electrophysiology: which is highly invasive, low-throughput, and difficult to interpret. Optical recording of the membrane potential using voltage-sensitive fluorescent indicators provides an attractive alternative to probing Vm and AP dynamics in multiple cells, in monolayers, or three-dimensional tissue.BioTracker fVF2 Voltage Sensitive Dye readily detects fast changes in membrane potential in mammalian neurons and cardiomyocytes. The BioTracker dye combines a xanthene-based dye as a fluorescent reporter and a conjugated molecular wire that localizes the indicator to the cell membrane and facilitates photo-induced electron transfer (PeT) from an electron-rich aniline donor to the fluorophore within the low-dielectric environment of the lipid bilayer. The BioTracker dye displays a substantially reduced phototoxicity in cardiomyocytes relative to that of VF2.1.Cl, allowing for prolonged, continuous measurement of cardiomyocyte activity. The dye also exhibits adequate sensitivity and excellent brightness in cells for reporting AP waveforms in neurons and cardiomyocytes with high SNRs.Spectral Properties:Absorption: 520 nmEmission: 535 nmResearch Category:Cardiovascular DiseaseDevelopmental NeuroscienceLive Cell Dye

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