SCR544
人STEMCCA组成型多顺反子（OKSM）慢病毒重编程试剂盒
The Human STEMCCA Constitutive Polycistronic Lentivirus Kit contains high titer polycistronic lentivirus & Polybrene transfection reagent that have been validated for the generation of human iPS cells from human foreskin fibroblasts.
登录查看公司和协议定价
所有图片(1)
About This Item
UNSPSC代码:
12352207
eCl@ss:
32161000
NACRES:
NA.71
质量水平
制造商/商品名称
STEMCCA
技术
cell culture | stem cell: suitable
输入
sample type induced pluripotent stem cell(s)
运输
dry ice
一般描述
人STEMCCA组成型多顺反子（OKSM）慢病毒试剂盒包含高滴度多顺反子（OKSM）慢病毒和聚凝胺转染试剂，已经过验证可用于从人包皮成纤维细胞（HFF）生成人诱导多能干细胞。 STEMCCA载体由转录因子人Oct-4、Klf4、SOX-2和c-Myc（OKSM）组成，由自切割2A肽和IRES序列隔开（Sommer CA，2009）。 使用单个多顺反子慢病毒载体可显著提高重编程效率并减少病毒整合的数量。
组分
1. 人STEMCCA组成型（OKSM）慢病毒： （部件号SCR544-1）两（2）瓶EF1α-hSTEMCCA （OKSM）慢病毒（部件号CS204502）。 每瓶包含15 µL高滴度慢病毒。
2. 聚凝胺转染试剂： （部件号TR-1003-50UL）一（1）瓶包含50 µL 10 mg/mL储备液。
2. 聚凝胺转染试剂： （部件号TR-1003-50UL）一（1）瓶包含50 µL 10 mg/mL储备液。
质量
测试以确认从p6人包皮成纤维细胞生成iPS细胞。 尚未测试其他细胞类型，因此无法保证相似的结果。
储存分类代码
12 - Non Combustible Liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
监管及禁止进口产品
Frank Griscelli et al.
Oncotarget, 10(28), 2693-2708 (2019-05-21)
Recent development of cell reprogramming technologies brought a major hope for future cell therapy applications by the use of these cells or their derivatives. For this purpose, one of the major requirements is the absence of genomic alterations generating a
Yang Yu et al.
Scientific reports, 5, 10114-10114 (2015-05-13)
Human pluripotent stem cells, including cloned embryonic and induced pluripotent stem cells, offer a limitless cellular source for regenerative medicine. However, their derivation efficiency is limited, and a large proportion of cells are arrested during reprogramming. In the current study
Jun-Quan Li et al.
Molecular medicine reports, 9(3), 837-842 (2014-01-09)
Myocardial infarction (MI) is an increasing medical problem; however, its pathogenesis has yet to be elucidated and more effective treatment strategies are required. Induced pluripotent stem cells (iPSCs) were recently successfully generated using human somatic cells transfected with four transcription
Areechun Sotthibundhu et al.
Stem cell research & therapy, 7(1), 166-166 (2016-11-17)
Cellular reprogramming is a stressful process, which requires cells to engulf somatic features and produce and maintain stemness machineries. Autophagy is a process to degrade unwanted proteins and is required for the derivation of induced pluripotent stem cells (iPSCs). However
Martin Madill et al.
Molecular brain, 10(1), 22-22 (2017-06-15)
Amyotrophic lateral sclerosis, a devastating neurodegenerative disease, is characterized by the progressive loss of motor neurons and the accumulation of misfolded protein aggregates. The latter suggests impaired proteostasis may be a key factor in disease pathogenesis, though the underlying mechanisms
商品
Fibroblast growth factors (FGFs) regulate developmental pathways and mesoderm/ectoderm patterning in early embryonic development.
实验方案
产生人诱导多能干细胞（iPSC）的干细胞重编程实验方案，包括基于病毒和非病毒RNA的方法。
Stem cell reprogramming protocols to generate human induced pluripotent stem cells (iPSCs) including viral and non-viral RNA based methods.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门