产品名称
Quantitative Alkaline Phosphatase ES Characterization Kit, This Quantitative Alkaline Phosphatase Embryonic Stem (ES) Characterization Kit easily quantifies the amount of alkaline phosphatase present within their embryonic stem cell cultures using a convenient 96-well colorimetric enzymatic assay.
species reactivity (predicted by homology)
mammals
manufacturer/tradename
Chemicon®
technique(s)
cell culture | stem cell: suitable
input
sample type: human embryonic stem cell(s)
sample type cardiac stem cell(s)
sample type neural stem cell(s)
sample type hematopoietic stem cell(s)
sample type epithelial cells
sample type mesenchymal stem cell(s)
sample type pancreatic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)
Quality Level
Application
Stem Cell Research
Disclaimer
General description
Alkaline phosphatase (ALP) is a hydrolase enzyme responsible for dephosphorylating molecules such as nucleotides, proteins, and alkaloids under alkaline conditions. The enzyme is present within all tissues of the body but is elevated in cells of the liver, kidney, bone, placenta, embryo and under specific disease states. Under alkaline conditions (pH>10), ALP can catalyze the hydrolysis of p-nitrophenylphosphate (p-NPP) into phosphate and p-nitrophenol, a yellow colored by-product of the catalytic reaction. The amount of p-nitrophenol produced is proportional to the amount of alkaline phosphatase present within the reaction. The amount of ALP can thus be reliably quantified by reading the amount of p-nitrophenol amassed after the catalytic reaction at 405 nm on a spectrophotometer.
Other Notes
p-NPP Buffer: (Part No. ES011-10ML) 10 mL
Reaction Stop Solution: (Part No.CS200653) 5 mL
1X Wash Solution: (Part No. CS200652) 2 X 125 mL
Recombinant Alkaline Phosphatase Standard: (Part No. CS200654) 0.5 μg (10 μg/mL)
Preparation Note
Legal Information
signalword
Danger
hcodes
Hazard Classifications
Aquatic Chronic 3 - Eye Dam. 1 - Met. Corr. 1 - Repr. 2 - Skin Corr. 1B
存储类别
8A - Combustible corrosive hazardous materials
法规信息
相关内容
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