HCC95 Human Lung Squamous Carcinoma Cell Line

Lung cancer is the leading cause of cancer death, causing 23% of all cancer mortality in 2020. Among all lung cancer cases, 85% are categorized as non-small cell lung cancer (NSCLC) which is a heterogeneous group of diseases with poor prognosis and a 5-year survival rate of less than 20%. Among all NSCLC cases, 30-40% are categorized as lung squamous carcinoma (LUSC) which often affects the main airways and bronchi and is attributed to smoking as its principal etiology.

The HCC95 cell line is a human lung squamous cell carcinoma line, collected from a metastatic site of pleural effusion. Recent transcriptomic characterizations show that HCC95 show high similarity to the typical LUSC transcriptome.



Source
HCC95 human squamous lung carcinoma cells were derived from a 65-year-old male with NSCLC.

References
1.J Natl Compr Canc Netw. 2013, 11(6): 645–653 DOI: 10.6004/jnccn.2013.0084
2.Nat Rev Cancer 2014, 14(8): 535–546 DOI: 10.1038/nrc3775
3.J Thorac Dis. 2013, 5(Suppl 5): S463–S478 DOI: 10.3978/j.issn.2072-1439.2013.08.43
4.J Clin Oncol. 2010, 28(36): 5311–5320 DOI: 10.1200/JCO.2010.28.8126
5.Clin Can Res. 1999, 5(5): 991-1000
6.Nat Commun. 2019, 10(1): 3574 DOI: 10.1038/s41467-019-11415-2
7.J Cancer Res Clin Oncol 2010, 136(4): 493–502 DOI: 10.1007/s00432-009-0681-6

Among the NSCLC cell lines, HCC95 has been shown to retain the lung squamous carcinoma (LUSC) disease phenotype. Early research showed that HCC95 is very similar to the original tumor genetically and histochemically, with poor differentiation and formation of keratinization. Collectively, these demonstrate that HCC95 is a valuable cell line for modeling LUSC with high fidelity in vitro. A 2010 study has exemplified its use in studying NSCLC sensitivity to cisplatin treatment.

HCC95 cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and function.

This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.