HCC827 GR6 Human Lung Adenocarcinoma Cell Line

Lung cancer is the most common cause of cancer-related death worldwide. The major etiological factor of lung cancer is tobacco smoke, a complex mixture of many carcinogens that play a role in the genetic and epigenetic changes occurring in the respiratory

HCC827 GR6 human lung adenocarcinoma cell line is a model for gefitinib-resistant non-small cell lung cancer. Lung cancer is the most common cause of cancer-related death worldwide. Tobacco smoke, the main causal factor of lung cancer, is a complex mixture of many carcinogenic agents that effect genetic and epigenetic mutations in the respiratory epithelium during carcinogenesis.1Patient outcome depends on multiple factors, including the genomic signature of the tumor, and these factors help to identify patients most likely to benefit from targeted therapeutics. Patients with or EGFR (epidermal growth factor receptor, a tyrosine kinase) - activating mutations have a higher likelihood to respond to first-generation EGFR-targeted tyrosine kinase inhibitors such as erlotinib and gefitinib; however, Intrinsic or acquired resistance often limits the efficacy of targeted therapies. Resistance most often arises through secondary mutations in EGFR or amplification of the MET oncogene.2
The lung adenocarcinoma HCC827 is non-small cell lung cancer (NSCLC) cell line with an acquired E746-A750 deletion in the EGFR tyrosine kinase exon 19 domain.1 HCC827 has been used extensively in biomedical discovery and research. HCC827 GR6 is a gefitinib-resistant clone of the parental gefintinib-hypersensitive HCC827 cell line developed by six-month exposure to increasing concentrations of gefitinib in vitro.2 The resistance was shown to be a result of focal amplification of the MET proto-oncogene. Inhibition of MET signaling restores sensitivity of these cells to gefitinib. HCC827 GR6 is thus a relevant cellular model for studying resistance mechanisms for EGFR/ERBB-targeted therapeutics.

Source
HCC827 GR6 human lung adenocarcinoma cell line was derived from clonally isolated single cells of the parental HCC827 cell line. The parental cell line was isolated from non-small cell lung cancer tissue of a 39-year old female patient.1

References
1. Girard L, Zöchbauer-Müller S, Virmani AK, Gazdar AF, Minna JD. Cancer Res 2000; 60(17): 4894-4906.
2. Engelman JA et al. Science 2007; 316(5827): 1039-1043.

HCC827 GR6 is thus a relevant cellular model for studying resistance mechanisms for EGFR/ERBB-targeted therapeutics.

Cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

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