Py2T Mouse Mammary Tumor Cell Line

Py2T is a stable murine breast cancer cell line derived from the mammary tumor of MMTV-PyMT (mouse mammary tumor virus-polyoma middle tumor-antigen) transgenic mouse. PyMT is an oncogene which transforms cells in culture and leads to formation of mammary tumors in MMTV-PyMT mice by interacting with non receptor tyrosine kinase, c-Src.
These cells have a uniform cobblestone-like morphology, typical of differentiated epithelial cells, and express estrogen receptor (ER), and both basal (CK14) and luminal (CK8/18) markers. When these cells are implanted orthotopically in syngeneic FVB/N mice, they form non-metastatic invasive tumors with low or absent E-cadherin.
Epithelial-mesenchymal transition (EMT) is a process involved in the initiation of the invasion-metastasis cascade of cancer cells. 90% of breast cancer-related deaths are ascribed to systemic dissemination of cancer cells leading to their metastatic outgrowth in distant organs.


Source
The Py2T cell line was derived from a mammary tumor of a MMTV-PyMT transgenic mouse.

References
1.PLoS One 2012; 7(11):e48651. PMID: 23144919.
2.Oncogene 2015; 34(32):4190-4198. PMID: 25362852.
3.Sci Rep 2018; 8(1):12123. PMID: 30108334.
4.Ayse NK. University of Basel 2017; Dissertation. https://edoc.unibas.ch/59030/1/Ph.D.%20Dissertation.pdf

Py2T undergo epithelial-mesenchymal transition (EMT) in response to TGFβ in vitro. Long term treatment with TGFβ leads to formation of a mesenchymal subline of cells (Py2T LT) with elongated morphology expressing mesenchymal markers such as vimentin, fibronectin, and N-cadherin. Orthotopic implantation of Py2T LT cells has been reported to form microscopic lung metastatic lesions in mice. These cell lines will serve as a versatile model to investigate the molecular mechanisms underlying EMT.

Py2T cells should be stored in liquid nitrogen. The cells can be cultured for at least 10 passages without significantly affecting cell marker expression and function.

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