YUMM4.1 Mouse Melanoma Cell Line

The YUMM4.1 mouse melanoma cell line was derived from a 4-hydroxytamoxifen-induced melanoma tumor in a female C57/Bl/6 mouse into which mutations from the Braf/Pten genetically-engineered mouse model had been introduced via backcrossing. The YUMM4.1 cell line is homozygous negative for Pten and Cdkn2.1?

The YUMM cell lines recapitulate genetic drivers found in many human melanomas. YUMM4.1 is homozygous negative for Pten and Cdkn2+, and has no activating Braf mutations.

The promise of immune-based therapies in cancer and increasingly successful application of these approaches have emphasized the necessity of immune-competent models to evaluate immunological responses to cancer cells. Immunocompetent genetically-engineered mouse models with distinct genetic drivers of melanoma are essential for identifying potential immunotherapies, but are limited by the need to maintain colonies of multiple genotypes necessary to generate mouse models with appropriate genetic backgrounds.YUMM4.1 cells are syngenic with the immunocompetent C57BL/6 mouse background and retain genetic markers of the Braf/Pten mouse model. Although mutations in Braf V600 are prevalent in melanomas and are targets of specific drugs, first-line treatment of non-Braf mutant melanoma consists of immune checkpoint inhibitors, to which roughly half of patients do not respond. The YUMM4.1 cell line is homozygous negative for Pten and Cdkn2 but has no activating Braf mutations, making this cellular model ideal for investigating the biology of non-Braf-mutant melanomas and potential therapeutics for this difficult subset of cancer.​Mutation: Pten−/− Cdkn2−/−​ . Female.References:1. Meeth K et al. (2016) The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations. Pigment Cell Melanoma Res 29(5): 590-597.2. Dankort D et al. (2009) Braf(V600E) cooperates with Pten loss to induce metastatic melanoma. Nat Genet. 41(5): 544-552.

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

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