YUMM2.2 Mouse Melanoma Cell Line

The YUMM (Yale University Mouse Melanoma) cell lines were developed as models that recapitulate genetic drivers found in a significant proportion of human melanomas. YUMM2.2 cells are syngeneic with the immunocompetent C57Bl/6 mouse background.

The YUMM cell lines recapitulate genetic drivers found in many human melanomas. YUMM2.2 harbors a mutation that accelerates Braf/Pten-driven melanoma tumorigenesis and inactivates wt Pten and Cdkn2a.

The hope for immune-based therapies in cancer and recent progress in successful application of these approaches has focused research on the necessity for immunocompetent models to assess immune system responses to cancer cells. Immunocompetent genetically-engineered mouse models harboring discrete genetic drivers of melanoma are essential for studying potential immunotherapies, but are limited by the need to maintain colonies of multiple genotypes necessary to generate mouse models with appropriate genetic backgrounds.The YUMM2.2 cell line harbors a stabilized beta-catenin allele from excision of exon 3, a mutation that accelerates Braf/Pten-driven melanoma tumorigenesis, enhances metastasis, and controls tumor differentiation. Exon 3 excision is analogous to human beta-catenin stabilizing mutations found in human melanomas.SourceThe YUMM2.2 mouse melanoma cell line was derived from a 4-hydroxytamoxifen-induced melanoma tumor in a male C57BL/6 mouse into which mutations from the Braf/Pten genetically engineered mouse model had been introduced via backcrossing. References:1. Meeth K et al. (2016) The YUMM lines: a series of congenic mouse melanoma cell lines with defined genetic alterations. Pigment Cell Melanoma Res 29(5): 590-597.2. Dankort D et al. (2009) Braf(V600E) cooperates with Pten loss to induce metastatic melanoma. Nat Genet. 41(5): 544-552.

YUMM2.2 cells should be stored in liquid nitrogen until use. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting cell marker expression and functionality.

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