N27-A Rat Dopaminergic Neural Cell Line

N27-A is a clonal derivative of N27 characterized by consistently high expression of the dopaminergic markers TH, dopamine transporter (DAT), and Tuj1 . N27-A cells express the dopaminergic neuron transcription factors Nurr1, En1, FoxA2, and Pitx3 as well as the monoamine transporter VMAT2, but do not express dopamine-beta-hydroxylase (DbH), the enzyme that converts dopamine to norepinephrine. N27-A cells exhibit higher sensitivity to neurotoxins as compared to parental N27 cells, and release dopamine under both basal and depolarization conditions. These features make the N27-A cell line an improved model for Parkinson′s disease research.
Source:
The N27-A cell line is a clonal derivative of the parental N27 cell line selected for high expression of dopaminergic neural markers. The parental cell line was originally isolated from dopaminergic neurons from an embryonic day 14 rat mesencephalon and immortalized with SV40 large T antigen .

Parkinson’s disease is characterized by the death of dopaminergic neurons in the substantia nigra of the brain . In vitro models are critical for understanding the molecular pathology of Parkinson’s disease. The N27 immortalized rat dopaminergic cell line has been widely utilized in Parkinson’s research for over 20 years. However long-term passaging has led the N27 cell line to become a mixture of cell types with widely variable expression of dopaminergic neural markers such as tyrosine hydroxylase (TH).

Neural Lineage Cells

N27-A rat dopaminergic neural cell line is a suitable model for Parkinson’s disease research.

Research Category
Neuroscience

Research Sub Category
Neurochemistry & Neurotrophins

Neurodegenerative Diseases

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• Each vial contains ≥ ≥1X10⁶ cells/vial.
• Cells are tested negative for infectious diseases by a Mouse/Rat Comprehensive CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are verified to be of rat origin and negative for inter-species contamination from mouse, chinese hamster, Golden Syrian hamster, human and non-human primate (NHP) as assessed by a Contamination CLEAR panel by Charles River Animal Diagnostic Services.
• Cells are negative for mycoplasma contamination.

Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.

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