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一般描述
GT1-7是永生化的成熟小鼠下丘脑GnRH神经元细胞系。 通过将含有与SV40 T抗原癌基因编码区偶联的GnRH基因启动子区的转基因引入转基因小鼠中来产生永生化的GnRH神经元1。 从一只小鼠中取出所得的下丘脑前部肿瘤，并将细胞解离并克隆。 GT1-7是成熟分化的GnRH神经元的克隆系，表现出高水平的Gnrh1 mRNA并响应去极化而分泌GnRH。
促性腺激素释放激素（GnRH）是一种神经肽，由下丘脑内的GnRH神经元合成和释放，是正常生殖发育和功能所必需的。下丘脑喙侧GnRH神经元的稀缺和分散分布使细胞的生物学研究变得困难。
细胞系描述
神经谱系细胞
应用
根据产品文件中详述的“限制使用协议”条款，本产品预期用于销售和仅用于内部非商业研究用途。有关任何其他用途的信息，请联系licensing@emdmillipore.com。
研究类别
内分泌
神经科学
内分泌
神经科学
质量
• 每瓶含有≥ 1X106 个活细胞。
•Charles River动物诊断服务通过小鼠/大鼠综合透明面板PCR面板对细胞进行传染病检测，结果为阴性。
•细胞对支原体污染检测呈阴性。
•Charles River动物诊断服务通过小鼠/大鼠综合透明面板PCR面板对细胞进行传染病检测，结果为阴性。
•细胞对支原体污染检测呈阴性。
储存及稳定性
储存在液氮中。在初始解冻后，细胞可以培养至少10代，而不会显著影响细胞标志物的表达和功能。
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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Proceedings of the National Academy of Sciences of the United States of America, 89(9), 4149-4153 (1992-05-01)
Mammalian reproduction is dependent upon intermittent delivery of luteinizing hormone-releasing hormone (LHRH) to the anterior pituitary. This mode of secretion is required to sensitize maximally the gonadotrophs to LHRH stimulation and to regulate gonadotropin gene expression. While LHRH secretion is
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An immortalized LHRH cell line has recently been developed by genetically targeting these neurons for tumorigenesis. One of the subclones, the GT1-7 cells, was characterized at both the light and electron microscopic levels to study the cellular and subcellular organization
A small-molecule degrader of TET3 as treatment for anorexia nervosa in an animal model.
Lv, et al.
Proceedings of the National Academy of Sciences of the USA, 120, e2300015120-e2300015120 (2023)
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Molecular endocrinology (Baltimore, Md.), 9(4), 467-477 (1995-04-01)
The molecular mechanisms specifying gene expression in individual neurons of the mammalian central nervous system have been difficult to study due to the cellular complexity of the brain and the absence of cultured model systems representing differentiated central nervous system
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Neuron, 5(1), 1-10 (1990-07-01)
By genetically targeting tumorigenesis to specific hypothalamic neurons in transgenic mice using the promoter region of the gonadotropin-releasing hormone (GnRH) gene to express the SV40 T-antigen oncogene, we have produced neuronal tumors and developed clonal, differentiated, neurosecretory cell lines. These
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