产品名称
AC16 人心肌细胞细胞系, AC16 Human Cardiomyocytes can be serially passaged and can differentiate when cultured in mitogen-free medium. The cells may be used to study developmental regulation of cardiomyocytes.
biological source
human
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
Quality Level
Analysis Note
• 每小瓶含有≥ 1X106个活细胞。
•通过PCR检测细胞，对HPV-16、HPV-18、甲型、乙型、丙型肝炎和HIV-1 & 2型病毒呈阴性。
•细胞对支原体污染呈阴性。
•通过STR分析对每批细胞进行基因分型，以验证细胞系的唯一身份。
•通过PCR检测细胞，对HPV-16、HPV-18、甲型、乙型、丙型肝炎和HIV-1 & 2型病毒呈阴性。
•细胞对支原体污染呈阴性。
•通过STR分析对每批细胞进行基因分型，以验证细胞系的唯一身份。
Application
研究类别
心脏
心血管疾病
毒性
心脏
心血管疾病
毒性
该产品可出售给学术机构或非营利性实体以供研究使用。有关商业许可的信息，请联系licensing@emdmillipore.com。
Biochem/physiol Actions
心肌细胞
Disclaimer
仅供研究使用。在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。
General description
AC16人心肌细胞系是一种增殖细胞系，源自成人心室心脏组织的原代细胞与SV40转化的尿苷营养缺陷型人成纤维细胞融合，不含线粒体DNA。 在无尿苷培养基中筛选去除未融合的成纤维细胞后，将融合细胞进一步亚克隆，然后筛选是否存在SV40大T-ag，β-肌球蛋白重链（βMHC）和连接蛋白43（CX-43）。
通过改变培养条件和沉默SV40 T-ag的表达可以控制AC16细胞的增殖和分化。 这些细胞可用于在正常发育过程中，并且在细胞、细胞器和分子水平的病理条件下研究心脏基因的表达和功能。
通过改变培养条件和沉默SV40 T-ag的表达可以控制AC16细胞的增殖和分化。 这些细胞可用于在正常发育过程中，并且在细胞、细胞器和分子水平的病理条件下研究心脏基因的表达和功能。
Preparation Note
AC16人心肌细胞株应保存在液氮中。在初始解冻后，细胞可以培养至少10代，而不会显著影响细胞标志物的表达和功能。
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
高风险级别生物产品-Merck
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