product name
AC16 人心肌细胞细胞系, AC16 Human Cardiomyocytes can be serially passaged and can differentiate when cultured in mitogen-free medium. The cells may be used to study developmental regulation of cardiomyocytes.
生物来源
human
质量水平
技术
cell culture | mammalian: suitable
运输
dry ice
一般描述
AC16人心肌细胞系是一种增殖细胞系，源自成人心室心脏组织的原代细胞与SV40转化的尿苷营养缺陷型人成纤维细胞融合，不含线粒体DNA。 在无尿苷培养基中筛选去除未融合的成纤维细胞后，将融合细胞进一步亚克隆，然后筛选是否存在SV40大T-ag，β-肌球蛋白重链（βMHC）和连接蛋白43（CX-43）。
通过改变培养条件和沉默SV40 T-ag的表达可以控制AC16细胞的增殖和分化。 这些细胞可用于在正常发育过程中，并且在细胞、细胞器和分子水平的病理条件下研究心脏基因的表达和功能。
通过改变培养条件和沉默SV40 T-ag的表达可以控制AC16细胞的增殖和分化。 这些细胞可用于在正常发育过程中，并且在细胞、细胞器和分子水平的病理条件下研究心脏基因的表达和功能。
细胞系描述
心肌细胞
应用
研究类别
心脏
心血管疾病
毒性
心脏
心血管疾病
毒性
该产品可出售给学术机构或非营利性实体以供研究使用。有关商业许可的信息，请联系licensing@emdmillipore.com。
质量
• 每小瓶含有≥ 1X106个活细胞。
•通过PCR检测细胞，对HPV-16、HPV-18、甲型、乙型、丙型肝炎和HIV-1 & 2型病毒呈阴性。
•细胞对支原体污染呈阴性。
•通过STR分析对每批细胞进行基因分型，以验证细胞系的唯一身份。
•通过PCR检测细胞，对HPV-16、HPV-18、甲型、乙型、丙型肝炎和HIV-1 & 2型病毒呈阴性。
•细胞对支原体污染呈阴性。
•通过STR分析对每批细胞进行基因分型，以验证细胞系的唯一身份。
储存及稳定性
AC16人心肌细胞株应保存在液氮中。在初始解冻后，细胞可以培养至少10代，而不会显著影响细胞标志物的表达和功能。
免责声明
仅供研究使用。在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
高风险级别生物产品-Merck
Vikram Thakur et al.
International journal of molecular sciences, 22(19) (2021-10-14)
Diabetes is a major risk factor for cardiovascular diseases, especially cardiomyopathy, a condition in which the smooth muscles of the heart become thick and rigid, affecting the functioning of cardiomyocytes, the contractile cells of the heart. Uncontrolled elevated glucose levels
P Litzkas et al.
Molecular and cellular biology, 4(11), 2549-2552 (1984-11-01)
A method for fusion of protoplasts bearing amplified plasmids and human diploid fibroblasts or other cell types in suspension is described. Transient expression of plasmid-encoded proteins occurs in up to 50% of the human cells, as demonstrated for simian virus
Vikram Thakur et al.
Biomolecules, 11(4) (2021-05-01)
Myocardial fibrosis is one of the major complications of long-term diabetes. Hyperglycemia induced cardiomyocyte atrophy is a frequent pathophysiological indicator of diabetic heart. The objective of this study was to investigate the cardioprotective effect of glycyrrhizin (GLC) on myocardial damage
Gayathri Narasimhan et al.
Clinical and experimental pharmacology & physiology, 46(8), 743-751 (2019-05-08)
Obg-like ATPase 1 (OLA1) that possesses both GTP and ATP hydrolyzing activities has been shown to be involved in translational regulation of cancer cell growth and survival. Also, GSK3β signalling has been implicated in cardiac development and disease. However, the
Lucia Scisciola et al.
Frontiers in pharmacology, 13, 868365-868365 (2022-06-04)
The cellular mechanisms involved in myocardial ischemia/reperfusion injury (I/R) pathogenesis are complex but attributable to reactive oxygen species (ROS) production. ROS produced by coronary endothelial cells, blood cells (e.g., leukocytes and platelets), and cardiac myocytes have the potential to damage
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